Browsing by Author "Shao, Chuyang"

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    Flavonoid-attracted Aeromonas sp. from the Arabidopsis root microbiome enhances plant dehydration resistance
    (2022) He, Danxia; Singh, Sunil K.; Peng, Li; Kaushal, Richa; Vílchez, Juan I.; Shao, Chuyang; Wu, Xiaoxuan; Zheng, Shuai; Morcillo, Rafael J. L.; Paré, Paul W. (TTU); Zhang, Huiming
    Flavonoids are stress-inducible metabolites important for plant-microbe interactions. In contrast to their well-known function in initiating rhizobia nodulation in legumes, little is known about whether and how flavonoids may contribute to plant stress resistance through affecting non-nodulating bacteria. Here we show that flavonoids broadly contribute to the diversity of the Arabidopsis root microbiome and preferentially attract Aeromonadaceae, which included a cultivable Aeromonas sp. H1 that displayed flavonoid-induced chemotaxis with transcriptional enhancement of flagellum biogenesis and suppression of fumarate reduction for smooth swims. Strain H1 showed multiple plant-beneficial traits and enhanced plant dehydration resistance, which required flavonoids but not through a sudden “cry-for-help” upon stress. Strain H1 boosted dehydration-induced H2O2 accumulation in guard cells and stomatal closure, concomitant with synergistic induction of jasmonic acid-related regulators of plant dehydration resistance. These findings revealed a key role of flavonoids, and the underlying mechanism, in mediating plant-microbiome interactions including the bacteria-enhanced plant dehydration resistance.
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    Rhizobacterium-derived diacetyl modulates plant immunity in a phosphate-dependent manner
    (2020) Morcillo, Rafael J.L.; Singh, Sunil K.; He, Danxia; An, Guo; Vílchez, Juan I.; Tang, Kai; Yuan, Fengtong; Sun, Yazhou; Shao, Chuyang; Zhang, Song; Yang, Yu; Liu, Xiaomin; Dang, Yashan; Wang, Wei; Gao, Jinghui; Huang, Weichang; Lei, Mingguang; Song, Chun Peng; Zhu, Jian Kang; Macho, Alberto P.; Paré, Pual W. (TTU); Zhang, Huiming
    Plants establish mutualistic associations with beneficial microbes while deploying the immune system to defend against pathogenic ones. Little is known about the interplay between mutualism and immunity and the mediator molecules enabling such crosstalk. Here, we show that plants respond differentially to a volatile bacterial compound through integral modulation of the immune system and the phosphate-starvation response (PSR) system, resulting in either mutualism or immunity. We found that exposure of Arabidopsis thaliana to a known plant growth-promoting rhizobacterium can unexpectedly have either beneficial or deleterious effects to plants. The beneficial-to-deleterious transition is dependent on availability of phosphate to the plants and is mediated by diacetyl, a bacterial volatile compound. Under phosphate-sufficient conditions, diacetyl partially suppresses plant production of reactive oxygen species (ROS) and enhances symbiont colonization without compromising disease resistance. Under phosphate-deficient conditions, diacetyl enhances phytohormone-mediated immunity and consequently causes plant hyper-sensitivity to phosphate deficiency. Therefore, diacetyl affects the type of relation between plant hosts and certain rhizobacteria in a way that depends on the plant's phosphate-starvation response system and phytohormone-mediated immunity.