Identification and characterization of molecular genetic differences among Bos indicus x Bos taurus: Feedlot cattle
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The objective of this study was to identify genotypic differences in different breeds of cattle and to determine their associations with growth, performance and carcass traits. The majority of cattle used in this study were Bos indicus influenced. All the cattle were identified for feedlot and carcass data such as initial weight (IWT), final weight (FWT), number of days on feed (DOF), average daily gain (ADG), skeletal mamrity (SM), overall maturity (OM), lean maturity (LM), hot carcass weight (HCW), calpastatin gene activity (CAL), rib-eye area REA), kidney, pelvic and heart fat percentage (KPH), marbling score (MRB), and Wamer-Bratzler shear force. Blood samples were obtained from all animals to extract genomic DNA. Five candidate genes were investigated for polymorphisms related to performance traits. These genes were Growth Hormone, Insulin-like Growth Factor 1, Somatostatin, Leptin and Prolactin. Associations between genotypes and performance measured were analyzed through analyses of variance procedures. Genotypic and allelic frequencies were calculated for the prolactin microsatellite marker in animals in the upper 10% and lower 10% of the distribution for all traits studied. Genotypes were not able to be determined for Growth Hormone, Insulin-like Growth Factor 1, Leptin, and Somatostatin.