Isolation frequency of xanthomonas axonopodis pv. malvacearum from acid delinted and easiflo treated cotton seed
Alexander, Aaron S.
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This project was conducted to determine if an alternative method of delinting cotton seed based on mechanical delinting and use of the Easiflo seed coating process, would be comparable to the current standard method of acid delinting seed in the frequency of survival of Xanthomonas axonopodis pv. malvacearum (Xam). Paymaster 2326 RR was grown at Halfway, TX in 2006 and treated with a solution of Xam + Silwet L77. In 2007, cotton bolls from Paymaster 2326RR and All-Tex Xpress RR were injected with concentrations of Xam. In 2007, bulk harvested seed from a third cultivar (Deltapine 164B2RF) was obtained from a field that had been naturally infected with Xam. Seed from each infection method and cultivar was divided into two batches, with one batch mechanically delinted and treated with Easiflo coating and the other batch processed with acid delinting. For each batch, isolations were made on 1,000 seed (900 in Paymaster 2326 RR from 2006) and yellow, mucoid colonies characerisitc of Xam were stored and later tested for pathogenicity on cotton seedlings. In 2006, Paymaster 2326RR (inoculated by sprays on leaves) had a higher frequency of Xam using Easiflo treatment (2.7%) than with the acid treatment (0%). In 2007, All-Tex Xpress RR (boll injection) had a higher frequency of Xam recovered from seed with acid delinted seed (3.8%) than with the Easiflo treatment (1.1%); however, isolation frequencies were similar for Paymaster 2326 RR. No differences in Xam isolation frequency were observed between the two methods for naturally infested seed. Overall, results from this study are inconclusive, thus, additional studies are required to better understand the impact of using the Easiflo seed coating system on Bacterial blight development.