Molecular characterization of the iron signaling pathway by plant growth promoting rhizobacteria GBO3

Date

2014-08

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Abstract

Bacillus subtilis GBO3 is a soil bacterium that regulates several plant responses related to growth and development. GBO3 emits an array of volatile organic compounds capable of affecting signal pathways including iron deficiency induced responses. GBO3 has been shown to increase iron accumulation by overexpressing the plant’s own iron uptake machinery, however the GBO3-activated signaling pathway operative in Arabidopsis has yet to be characterized. The aim of this work was to establish an experimental procedure to identify and characterize signaling intermediates in the inducible-iron signaling pathway via a forward genetics approach. A transgenic Arabidopsis line capable of GBO3-induced growth promotion and other iron related responses similar to Col-0, FIT1-LUC/cpl1-6, was utilized. An EMS mutagenized population of 3500 seeds was screened via luciferase bioluminescence to identify mutants with compromised GBO3-induced iron signaling. After several confirmation re-screens, a preliminary positive mutant, 1M14 that displayed a reduced luciferase bioluminescence phenotype was identified. Future studies will map the mutation location for gene identification.

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Keywords

GBO3 (bacterium), Arabidopsis, Iron signaling

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