Enhanced sensitive MS analysis of sulfated glycans

Date
2014-12
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract

Sulfated glycans widely exist in cells and tissues of human body. They are involved in many biological activities such as protein folding and degradation, cell growth, cell-cell interaction, and anticoagulation. They are also relevant to many human diseases. The most attractive is cancer metastasis. In recent studies, sulfated glycans have been selected as biomarkers for the diagnosis of many diseases. Thus a valid and sensitive method is urgently needed for the detection and characterization of sulfated glycans. So far there have been many techniques for analysis of sulfated glycans, such as isotopic labeling of S, nuclear magnetic resonance (NMR), electrophoresis and different type of mass spectrometry. Each method has its capability for analysis of sulfated glycans in some aspects. But there are always some limitations associated with sensitivity and stability. In order to achieve sensitive and stably quantitative detection, we introduced several strategies to modify sulfated glycans. In this thesis, two methods will be introduced to improve the analysis of sulfated glycans. One is using an ionic liquid (IL) as pair ion to interact with heparin derived sulfate glycans. This method is capable of highly increasing the ionization efficiency of sulfated glycans in positive ion mode electrospray ionization mass spectrometry (ESI-MS). The other method was using a single and double permethylation strategy to increase the hydrophobicity and proton affinity of sulfate N-glycans released from bovine thyroid stimulation hormone. This strategy improved liquid chromatography (LC)-MS analysis and detection of sulfated.

Description
Keywords
Sulfated glycan, Ionic liquid, Liquid chromatography mass spectrometry (LC-MS), Electrospray ionization mass spectrometry (ESI-MS), Quantitative analysis, Permethylation
Citation