Analyzing airborne environmental DNA: A comparison of extraction methods, primer type, and trap type on the ability to detect airborne eDNA from terrestrial plant communities

dc.creatorJohnson, Mark D.
dc.creatorCox, Robert D.
dc.creatorBarnes, Matthew A.
dc.date.accessioned2021-08-04T19:36:55Z
dc.date.available2021-08-04T19:36:55Z
dc.date.issued2019
dc.description© 2019 The Authors. Environmental DNA published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.en_US
dc.description.abstractAirborne environmental DNA (eDNA) research has typically focused on the detection of pollen from anemophilous terrestrial plant species; however, recent findings have expanded the definition of airborne eDNA to include a variety of eDNA sources, such as leaf and flower fragments. While methods for capturing pollen are well studied, there is less known about how to best analyze and capture more broadly defined bulk airborne eDNA samples. Therefore, this study aimed to identify efficient techniques for the extraction and amplification of airborne eDNA and how best to capture these samples. First, we collected samples using Big Spring Number Eight dust traps and quantified the influence of three different extraction methods (cetrimonium bromide-chloroform, DNeasy Plant Maxi Kit, and DNeasy PowerPlant Pro DNA Isolation Kit) and two global plant primer sets (targeting the trnL and ITS2 genes) on quantitative polymerase chain reaction (qPCR)-based terrestrial plant detection. Additionally, we examined how best to capture airborne eDNA by comparing the performance of three difference passive dust collectors (Big Spring Number Eight, Modified Wilson and Cooke, and Marble dust collectors). We found that the detection of airborne eDNA was significantly influenced by both the extraction methods employed and choice of qPCR primer. Additionally, we found that all three traps preformed similarly but the Big Spring Number Eight dust traps trended as consistently one of the most effective, shedding light on the nonhomogeneous nature of airborne eDNA. This study represents the first methodology paper focusing on airborne eDNA as a bulk environmental sample containing more than just pollen. In the future, additional research should examine more about the ecology of airborne eDNA (origin, state, transport, fate), explore active dust collectors compared to the passive traps used here, and develop the application of metabarcoding for airborne eDNA analysis.en_US
dc.identifier.citationJohnson, MD, Cox, RD, Barnes, MA. Analyzing airborne environmental DNA: A comparison of extraction methods, primer type, and trap type on the ability to detect airborne eDNA from terrestrial plant communities. Environmental DNA. 2019; 1: 176– 185. https://doi.org/10.1002/edn3.19en_US
dc.identifier.urihttps://doi.org/10.1002/edn3.19
dc.identifier.urihttps://hdl.handle.net/2346/87585
dc.language.isoengen_US
dc.subjectAiren_US
dc.subjecteDNAen_US
dc.subjectPollenen_US
dc.subjectqPCRen_US
dc.subjectVegetation Surveyen_US
dc.titleAnalyzing airborne environmental DNA: A comparison of extraction methods, primer type, and trap type on the ability to detect airborne eDNA from terrestrial plant communitiesen_US
dc.typeArticleen_US

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