Measurement of Gas Exchange on Excised Grapevine Leaves Does Not Differ from In Situ Leaves, and Potentially Shortens Sampling Time

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dc.creatorKar, Suraj
dc.creatorMontague, Thayne
dc.creatorVillanueva-Morales, Antonio
dc.creatorHellman, Edward
dc.date.accessioned2022-03-10T17:22:14Z
dc.date.available2022-03-10T17:22:14Z
dc.date.issued2021
dc.description© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).en_US
dc.description.abstractUse of leaf gas exchange measurement enhances the characterization of growth, yield, physiology, and abiotic stress response in grapevines. Accuracy of a crop response model depends upon sample size, which is often limited due to the prolonged time needed to complete gas exchange measurement using currently available infra-red gas analyzer systems. In this experiment, we measured mid-day gas exchange of excised and in situ leaves from field grown wine grape (Vitis vinifera) cultivars. Depending upon cultivar, we found measuring gas exchange on excised leaves under a limited time window post excision gives similar accuracy in measurement of gas exchange parameters as in situ leaves. A measurement within a minute post leaf excision can give between 96.4 and 99.5% accuracy compared to pre-excision values. When compared to previous field data, we found the leaf excision technique reduced time between consecutive gas exchange measurements by about a third compared to in situ leaves (57.52 ± 0.39 s and 86.96 ± 0.41 s, for excised and in situ, respectively). Therefore, leaf excision may allow a 50% increase in experimental sampling size. This technique could solve the challenge of insufficient sample numbers, often reported by researchers worldwide while studying grapevine leaf gas exchange using portable gas exchange systems under field conditions.en_US
dc.identifier.citationKar S, Montague T, Villanueva-Morales A, Hellman E. Measurement of Gas Exchange on Excised Grapevine Leaves Does Not Differ from In Situ Leaves, and Potentially Shortens Sampling Time. Applied Sciences. 2021; 11(8):3644. https://doi.org/10.3390/app11083644en_US
dc.identifier.urihttps://doi.org/10.3390/app11083644
dc.identifier.urihttps://hdl.handle.net/2346/88856
dc.language.isoengen_US
dc.subjectCO2 Assimilation Rateen_US
dc.subjectStomatal Conductanceen_US
dc.subjectMeasurement Timeen_US
dc.subjectPhotosynthetic Decline Curveen_US
dc.subjectInfra-Red Gas Analyzeren_US
dc.titleMeasurement of Gas Exchange on Excised Grapevine Leaves Does Not Differ from In Situ Leaves, and Potentially Shortens Sampling Timeen_US
dc.typeArticleen_US

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