Improving semen parameters through modification of semen collection/extension



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Texas Tech University


To date, the common thread in the use of semen extenders/collection techniques, whether used for fresh extended semen, chilled semen, or cryopreserved semen, is that the extenders have all traditionally added post-collection. The objective of this study was to determine if modifying the method of collection/extension of semen would improve semen parameters by lessening cold and pH shock.

Ten canine semen samples were collected with a modified artificial vagina to allow for a true split collection. The treatment half of the sample was collected into warmed extension media. The control half was collected into a dry container and no attempts to maintain temperature were used. Standard semen parameters along with the available sperm pool and number of inseminations were evaluated at specific time intervals and evaluations continued until the samples reached zero percent motility. Data analysis was performed with SPSS using the general linear model, Chi square and appropriate t-tests.

There was a difference between the treatment and control groups for Motility (P<.001), Motility by Time (P<.001), Time to Zero Motility (P<.001), Time to Last Full Insemination (P<.03), Forward Progression (P<.001), Acrosome Reaction (P<.001), Acrosome Reaction by Time (P<.017), and Viability (P<.001). There was no difference in morphology between the treatment and control groups (P>.062).

Modification of the semen collection/extension procedure resulted in improved semen parameters for extended periods of time post collection. The data suggest the improvement in semen parameters would result in improved quality and longevity of semen used for artificial insemination procedures and infertility treatment, possibly due to decreased effects of cold and pH shock. Further studies resulting in viable offspring will be needed to confirm these observations.



Spermatozoa -- Motility, Artificial insemination, Semen