Development of a liquid-liquid, solid-phase extraction (SPE), LC-MS/MS analytical method for qualitative and quantitative drug residue analysis in white-tailed deer



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In Texas, white-tailed deer farming is an established and growing industry. Owners and managers struggle with animal health problems within the deer industry; as a result, veterinary drugs are used. The administration of antibiotics for treatment of infectious diseases may lead to residues in tissues. The quality and safety of food from animal origin is a growing concern for public health agencies. It is important in the deer industry as well as in other food producing animals such as cattle, to monitor the use of veterinary drugs to minimize residues in tissues after slaughter. Residue analysis are based on qualitative and quantitative methods. These methods are used to determine the amount of compound present in a sample. The choice of analytical method of residue testing is based on the assessment of the benefits and disadvantages of the technique. Qualitative methods include, confirmation of the identity of the compound present while quantitative is the determination of the amount of the compound present. It is important to develop quantitative methods that are highly selective, sensitive, and reliable to accurately determine veterinary drugs in complex matrices. Currently, for quantitative and qualitative analysis the most powerful technique to use is liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS). In this dissertation, a brief synopsis of the methodology, instrumentation, sample preparation methods and critical parameters for an analytical method validation will be discussed, as well as, the work flow of liquid chromatography tandem mass spectrometry (LC-MS/MS) method development. LC-MS/MS was exploited for qualitative and quantitative analysis of veterinary drugs (florfenicol, chlortetracycline, tulathromycin, chloramphenicol, and ractopamine). To facilitate qualitative and quantitative analytical method development of veterinary drugs, a sample preparation method was developed to extract individual and multiple veterinary drugs, and an LC-MS/MS method was developed and validated to quantify these drugs. The methodology was applied to white-tailed deer tissues. This research will aid the white-tailed deer breeder with regulation and safety compliance.



Veterinary drug, White-tailed deer, LC-MS/MS