Development of Protoplast Regeneration System in Soybean

This study focuses on the use of protoplast culture and inducible gene expression systems for efficient plant regeneration and genetic modification in soybean (Glycine max). Protoplast culture, which involves cultivating plant cells without cell walls, provides a versatile tool for genome manipulation and research in genomics and transcriptomics. It allows for efficient delivery of exogenous DNA, RNA, and protein into cells, making it valuable for genetic engineering. The study highlights the benefits of protoplast culture in gene editing and its applications in studying gene function and transient gene analysis. The CRISPR/Cas9 system is discussed as a powerful tool for direct modification of crop genomes, enabling trait discovery and agricultural improvement. The role of developmental regulators, such as hormones and transcription factors, in plant regeneration is explored. Hormones like auxins and cytokinins are crucial for promoting cell division, organogenesis, and rooting in plants. Transcription factors, when overexpressed, enhance the efficiency of plant regeneration. However, careful modulation of their expression levels is necessary to avoid detrimental effects on plant development. Inducible gene expression systems, particularly chemical-inducible systems, offer precise control over gene expression in response to external stimuli. The use of inducers like dexamethasone and estradiol effectively regulates gene expression in soybean and other plant species. In conclusion, this thesis highlights the potential of protoplast culture and inducible gene expression systems for efficient plant regeneration and genetic modification in soybean. It emphasizes the importance of balancing developmental regulators and utilizing precise control over gene expression to optimize regeneration outcomes.

Embargo status: Restricted until 09/2025. To request the author grant access, click on the PDF link to the left.