A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)

Simple item page
dc.creatorShah, Sujit
dc.creatorChand, Krishna
dc.creatorRekadwad, Bhagwan
dc.creatorShouche, Yogesh S.
dc.creatorSharma, Jyotsna
dc.creatorPant, Bijaya
dc.date.accessioned2021-05-11T20:28:23Z
dc.date.available2021-05-11T20:28:23Z
dc.date.issued2021
dc.description.abstractBackground A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.en_US
dc.identifier.citationShah, S., Chand, K., Rekadwad, B. et al. A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata). BMC Biotechnol 21, 16 (2021). https://doi.org/10.1186/s12896-021-00676-9en_US
dc.identifier.urihttps://doi.org/10.1186/s12896-021-00676-9
dc.identifier.urihttps://hdl.handle.net/2346/86933
dc.language.isoengen_US
dc.publisherBMCen_US
dc.subjectEndophytesen_US
dc.subjectIndole Acetic Acid (IAA)en_US
dc.subjectOrchiden_US
dc.subjectVanda cristataen_US
dc.subjectSymbiosisen_US
dc.titleA prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)en_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
sharma_article.pdf
Size:
1.11 MB
Format:
Adobe Portable Document Format
Description:
Main article with TTU Libraries cover page
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.57 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Faculty Research