Altered O-glycomes of Renal Brush-Border Membrane in Model Rats with Chronic Kidney Diseases

dc.creatorYu, Aiying (TTU)
dc.creatorZhao, Jingfu (TTU)
dc.creatorZhong, Jieqiang (TTU)
dc.creatorWang, Junyao (TTU)
dc.creatorYadav, Shiv Pratap S.
dc.creatorMolitoris, Bruce A.
dc.creatorWagner, Mark C.
dc.creatorMechref, Yehia (TTU)
dc.date.accessioned2022-08-31T15:55:27Z
dc.date.available2022-08-31T15:55:27Z
dc.date.issued2021
dc.description© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).en_US
dc.description.abstractChronic kidney disease (CKD) is defined as a decrease in renal function or glomerular filtration rate (GFR), and proteinuria is often present. Proteinuria increases with age and can be caused by glomerular and/or proximal tubule (PT) alterations. PT cells have an apical brush border membrane (BBM), which is a highly dynamic, organized, and specialized membrane region containing multiple glycoproteins required for its functions including regulating uptake, secretion, and signaling dependent upon the physiologic state. PT disorders contribute to the dysfunction observed in CKD. Many glycoprotein functions have been attributed to their N- and O-glycans, which are highly regulated and complex. In this study, the O-glycans present in rat BBMs from animals with different levels of kidney disease and proteinuria were characterized and analyzed using liquid chromatography tandem mass spectrometry (LC–MS/MS). A principal component analysis (PCA) documented that each group has distinct O-glycan distributions. Higher fucosylation levels were observed in the CKD and diabetic groups, which may contribute to PT dysfunction by altering physiologic glycoprotein interactions. Fucosylated O-glycans such as 1-1-1-0 exhibited higher abundance in the severe proteinuric groups. These glycomic results revealed that differential O-glycan expressions in CKD progressions has the potential to define the mechanism of proteinuria in kidney disease and to identify potential therapeutic interventions.en_US
dc.identifier.citationYu A, Zhao J, Zhong J, Wang J, Yadav SPS, Molitoris BA, Wagner MC, Mechref Y. Altered O-glycomes of Renal Brush-Border Membrane in Model Rats with Chronic Kidney Diseases. Biomolecules. 2021; 11(11):1560. https://doi.org/10.3390/biom11111560en_US
dc.identifier.urihttps://doi.org/10.3390/biom11111560
dc.identifier.urihttps://hdl.handle.net/2346/90094
dc.language.isoengen_US
dc.subjectO-glycanen_US
dc.subjectBrush-Border Membraneen_US
dc.subjectProteinuria and Hypertensionen_US
dc.subjectObese and Diabeticen_US
dc.subjectChronic Kidney Diseaseen_US
dc.subjectDifferential Expression Analysisen_US
dc.subjectLC-MS/MSen_US
dc.titleAltered O-glycomes of Renal Brush-Border Membrane in Model Rats with Chronic Kidney Diseasesen_US
dc.typeArticleen_US

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