Functional analysis of select Arabidopsis ABA INSENSITIVE1/2-Like protein phosphatases

Simple item page
dc.contributor.committeeChairRock, Chris
dc.creatorZhang, Tiantian
dc.date.available2012-06-01T15:38:52Z
dc.date.issued2008-05
dc.degree.departmentBiology
dc.description.abstractWe have utilized a maize protoplast transient gene expression system to investigate the possible roles of several Arabidopsis protein phosphatase type 2C (PP2Cs), which are predicted to be involved in ABA responses by hierarchical clustering meta-analysis of transcriptome profiling datasets and sequence homologies to known PP2C effector ABA INSENSITIVE1. We recombined Cre-lox UPS host acceptor vectors (pCR701-705) containing the maize Ubiquitin promoter and N-terminal epitope tags with pUNI donor vectors containing various full length Arabidopsis PP2C cDNAs. On the basis of my maize protoplast transient expression data that suggested ABA antagonist effects of several overexpressed PP2Cs, I performed a reverse-genetic screen for AP2C9, AP2C12 and AP2C15 T-DNA insertion mutants and found supportive evidence from physiological assays on seed germination and root growth that these PP2Cs may be involved in ABA signaling. I conclude that the utilization of the maize protoplast transient expression system for moderate-throughput functional screening and systems-approach classification of gene activities can contribute to elucidation of plant abscisic acid (ABA) signal transduction pathways when combined with complimentary genetic and physiological approaches.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/2346/10362
dc.language.isoeng
dc.rights.availabilityUnrestricted.
dc.subjectTransfer deoxyribonucleic acid (T-DNA) Insertion Mutant
dc.subjectProtoplast
dc.subjectProtien phosphatase 2C (PP2C)
dc.subjectAbscisic acid (ABA)
dc.subjectBioinformatics
dc.titleFunctional analysis of select Arabidopsis ABA INSENSITIVE1/2-Like protein phosphatases
dc.typeThesis
thesis.degree.departmentBiology
thesis.degree.departmentBiological Sciences
thesis.degree.disciplineBiology
thesis.degree.grantorTexas Tech University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Zhang_Tiantian_Thesis.pdf
Size:
2.98 MB
Format:
Adobe Portable Document Format
Download

Collections

Electronic Theses and Dissertations