Separation of permethylated o-glycans, free oligosaccharides, and glycosphingolipid-glycans using porous graphitized carbon (Pgc) column

dc.creatorCho, Byeong Gwan (TTU)
dc.creatorPeng, Wenjing (TTU)
dc.creatorMechref, Yehia (TTU)
dc.date.accessioned2023-04-14T18:22:25Z
dc.date.available2023-04-14T18:22:25Z
dc.date.issued2020
dc.description© 2020 by the authors. Licensee MDPI, Basel, Switzerland. cc-by
dc.description.abstractGlycosylation is one of the most common and complex post-translational modifications of proteins. However, there are other carbohydrates such as free oligosaccharides and glycosphingolipids-glycans that are associated with important biological and clinical roles. To analyze these molecules using liquid chromatography coupled with mass spectrometry (LC-MS), the permethylation approach was utilized. Although permethylation is a commonly utilized glycan derivatization technique, separation of permethylated glycans released from glycosphingolipid (GSL) by LC-MS has never been previously demonstrated. Here, a nanoflow porous graphitized carbon (PGC) column coupled with a high-resolution mass spectrometer was used to achieve isomeric separation of these permethylated glycans. We demonstrate the separation of free reducing end and reduced end O-glycans, free oligosaccharides derived from human milk, and GSL glycans derived from the MDA-MB-231BR cancer cell line using PGC-LC-MS.
dc.identifier.citationCho, B.G., Peng, W., & Mechref, Y.. 2020. Separation of permethylated o-glycans, free oligosaccharides, and glycosphingolipid-glycans using porous graphitized carbon (Pgc) column. Metabolites, 10(11). https://doi.org/10.3390/metabo10110433
dc.identifier.urihttps://doi.org/10.3390/metabo10110433
dc.identifier.urihttps://hdl.handle.net/2346/92823
dc.language.isoeng
dc.subjectFree oligosaccharides
dc.subjectGlycomics
dc.subjectGlycosphingolipids
dc.subjectLC-MS
dc.subjectO-glycans
dc.subjectPGC
dc.titleSeparation of permethylated o-glycans, free oligosaccharides, and glycosphingolipid-glycans using porous graphitized carbon (Pgc) column
dc.typeArticle

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Main article with TTU Libraries cover page.pdf
Size:
3.55 MB
Format:
Adobe Portable Document Format

Collections