Impact of treatment strategies on cephalosporin and tetracycline resistance gene quantities in the bovine fecal metagenome

dc.creatorKanwar, Neena
dc.creatorScott, H. Morgan
dc.creatorNorby, Bo
dc.creatorLoneragan, Guy H. (TTU)
dc.creatorVinasco, Javier
dc.creatorCottell, Jennifer L.
dc.creatorChalmers, Gabhan
dc.creatorChengappa, Muckatira M.
dc.creatorBai, Jianfa
dc.creatorBoerlin, Patrick
dc.date.accessioned2023-05-24T20:23:29Z
dc.date.available2023-05-24T20:23:29Z
dc.date.issued2014
dc.descriptioncc-by-nc-nd
dc.description.abstractThe study objective was to determine the effects of two treatment regimens on quantities of ceftiofur and tetracycline resistance genes in feedlot cattle. The two regimens were ceftiofur crystalline-free acid (CCFA) administered to either one or all steers within a pen and subsequent feeding/not feeding of therapeutic doses of chlortetracycline. A 26-day randomized controlled field trial was conducted on 176 steers. Real-time PCR was used to quantify bla CMY-2, blaCTX-M, tet(A), tet(B), and 16S rRNA gene copies/gram of feces from community DNA. A significant increase in ceftiofur resistance and a decrease in tetracycline resistance elements were observed among the treatment groups in which all steers received CCFA treatment, expressed as gene copies/gram of feces. Subsequent chlortetracycline administration led to rapid expansion of both ceftiofur and tetracycline resistance gene copies/gram of feces. Our data suggest that chlortetracycline is contraindicated when attempting to avoid expansion of resistance to critically important third-generation cephalosporins.
dc.identifier.citationKanwar, N., Scott, H.M., Norby, B., Loneragan, G.H., Vinasco, J., Cottell, J.L., Chalmers, G., Chengappa, M.M., Bai, J., & Boerlin, P.. 2014. Impact of treatment strategies on cephalosporin and tetracycline resistance gene quantities in the bovine fecal metagenome. Scientific Reports, 4. https://doi.org/10.1038/srep05100
dc.identifier.urihttps://doi.org/10.1038/srep05100
dc.identifier.urihttps://hdl.handle.net/2346/93697
dc.language.isoeng
dc.titleImpact of treatment strategies on cephalosporin and tetracycline resistance gene quantities in the bovine fecal metagenome
dc.typeArticle

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Main article with TTU Libraries cover page.pdf
Size:
1.51 MB
Format:
Adobe Portable Document Format

Collections