Engineered Chimera Protein Constructs to Facilitate the Production of Heterologous Transmembrane Proteins in E. coli

dc.creatorOgunbowale, Adeyemi (TTU)
dc.creatorGeorgieva, Elka R. (TTU)
dc.date.accessioned2024-03-18T18:45:55Z
dc.date.available2024-03-18T18:45:55Z
dc.date.issued2024
dc.description© 2024 by the authors. cc-by
dc.description.abstractTo delve into the structure–function relationship of transmembrane proteins (TMPs), robust protocols are needed to produce them in a pure, stable, and functional state. Among all hosts that express heterologous TMPs, E. coli has the lowest cost and fastest turnover. However, many of the TMPs expressed in E. coli are misfolded. Several strategies have been developed to either direct the foreign TMPs to E. coli’s membrane or retain them in a cytosolic soluble form to overcome this deficiency. Here, we summarize protein engineering methods to produce chimera constructs of the desired TMPs fused to either a signal peptide or precursor maltose binding protein (pMBP) to direct the entire construct to the periplasm, therefore depositing the fused TMP in the plasma membrane. We further describe strategies to produce TMPs in soluble form by utilizing N-terminally fused MBP without a signal peptide. Depending on its N- or C-terminus location, a fusion to apolipoprotein AI can either direct the TMP to the membrane or shield the hydrophobic regions of the TMP, maintaining the soluble form. Strategies to produce G-protein-coupled receptors, TMPs of Mycobacterium tuberculosis, HIV-1 Vpu, and other TMPs are discussed. This knowledge could increase the scope of TMPs’ expression in E. coli.
dc.identifier.citationOgunbowale, A., & Georgieva, E.R.. 2024. Engineered Chimera Protein Constructs to Facilitate the Production of Heterologous Transmembrane Proteins in E. coli. International Journal of Molecular Sciences, 25(4). https://doi.org/10.3390/ijms25042354
dc.identifier.urihttps://doi.org/10.3390/ijms25042354
dc.identifier.urihttps://hdl.handle.net/2346/97736
dc.language.isoeng
dc.subjectE. coli expression host of heterologous transmembrane proteins
dc.subjectprotein engineering
dc.subjectsoluble transmembrane proteins
dc.subjecttransmembrane protein fusion strategies
dc.titleEngineered Chimera Protein Constructs to Facilitate the Production of Heterologous Transmembrane Proteins in E. coli
dc.typeReview

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Main article with TTU Libraries cover page.pdf
Size:
2.67 MB
Format:
Adobe Portable Document Format

Collections