N-glycomics of cerebrospinal fluid: Method comparison

Simple item page
dc.creatorCho, Byeong Gwan (TTU)
dc.creatorGutierrez Reyes, Cristian D. (TTU)
dc.creatorMechref, Yehia (TTU)
dc.date.accessioned2023-05-11T18:50:00Z
dc.date.available2023-05-11T18:50:00Z
dc.date.issued2021
dc.description© 2021 by the authors. Licensee MDPI, Basel, Switzerland. cc-by
dc.description.abstractCerebrospinal fluid (CSF) contains valuable biological and neurological information. However, its glycomics analysis is hampered due to the low amount of protein in the biofluid, as has been demonstrated by other glycomics studies using a substantial amount of CSF. In this work, we investigated different N-glycan sample preparation approaches to develop a more sensitive method. These methods, one with an increased amount of buffer solution during the N-glycan release step with a lower amount of sample volume and the other with Filter-Aided N-Glycan Separation (FANGS), were compared with recent work to demonstrate their effectiveness. It was demonstrated that an increased amount of buffer solution showed higher intensity in comparison to the previously published method and FANGS. This suggested that digestion efficiency during the N-glycan release step was not in an optimal condition from the previously published method, and that there is a substantial loss of sample with FANGS when preparing N-glycans from CSF.
dc.identifier.citationCho, B.G., Gutierrez, Reyes, C.D., & Mechref, Y.. 2021. N-glycomics of cerebrospinal fluid: Method comparison. Molecules, 26(6). https://doi.org/10.3390/molecules26061712
dc.identifier.urihttps://doi.org/10.3390/molecules26061712
dc.identifier.urihttps://hdl.handle.net/2346/93500
dc.language.isoeng
dc.subjectCerebrospinal fluid
dc.subjectGlycomics
dc.subjectLC-MS
dc.subjectPermethylation
dc.titleN-glycomics of cerebrospinal fluid: Method comparison
dc.typeArticle

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
mechref_article.pdf
Size:
1.43 MB
Format:
Adobe Portable Document Format
Description:
Main article with TTU Libraries cover page
Download

Collections

Faculty Research