The influence of beef quality characteristics on the internalization and thermal susceptibility of shiga toxin-producing Escherichia coli (STEC) in blade-tenderized beef steaks

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dc.contributor.committeeChairBrooks, J. Chance
dc.contributor.committeeMemberBrashears, Mindy M.
dc.contributor.committeeMemberEcheverry, Alejandro
dc.creatorCorliss, Blaine
dc.date.accessioned2015-01-21T15:12:54Z
dc.date.available2015-01-21T15:12:54Z
dc.date.issued2014-12
dc.description.abstractThe risk of Shiga toxin-producing Escherichia coli (STEC) survival in blade-tenderized beef is a concern for beef processors. This study evaluated the internalization and post-cooking survival of individual STEC serogroups (O157:H7, O26, O45, O103, O111, O121, and O145) in blade-tenderized beef steaks with different quality traits. Strip loins representing four combinations of USDA Quality Grade (Choice or Select) and pH category (High pH or Normal pH ) were inoculated (log CFU/cm2 attachment) with individual STEC serogroups before storage (14 d), blade tenderization, and cooking (50, 60, 71, or 85°C). Serogroup populations on raw steak surfaces and internal cores were determined. Rapid-based methods were used to detect the internal presence of STEC in cooked steaks. Internalization and post-cooking survival varied among STECs. All serogroups, except O45 and O121, were detected in the internal cores of steaks cooked to 50°C, while O103, O111, and O145 STEC were detected in steaks cooked to 50, 60, and 71°C.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/2346/60669
dc.rights.availabilityUnrestricted.
dc.subjectBeef
dc.subjectBlade tenderized
dc.subjectCooking
dc.subjectInternalization
dc.subjectNon-intact
dc.subjectShiga toxin-producing Escherichia coli (STEC)
dc.titleThe influence of beef quality characteristics on the internalization and thermal susceptibility of shiga toxin-producing Escherichia coli (STEC) in blade-tenderized beef steaks
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentAnimal and Food Science
thesis.degree.disciplineAnimal Science
thesis.degree.grantorTexas Tech University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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