A functionally important hydrogen-bonding network at the βDP/αDP interface of ATP synthase

dc.creatorMao, Hui Z. (TTU)
dc.creatorAbraham, Christopher G. (TTU)
dc.creatorKrishnakumar, Arathianand M. (TTU)
dc.creatorWeber, Joachim (TTU)
dc.date.accessioned2023-09-01T16:40:19Z
dc.date.available2023-09-01T16:40:19Z
dc.date.issued2008
dc.descriptioncc-by
dc.description.abstractATP synthase uses a unique rotary mechanism to couple ATP synthesis and hydrolysis to transmembrane proton translocation. The F1 subcomplex has three catalytic nucleotide binding sites, one on each β subunit, at the interface to the adjacent α subunit. In the x-ray structure of F 1 (Abrahams, J. P., Leslie, A. G. W., Lutter, R., and Walker, J. E. (1994) Nature 370, 621-628), the three catalytic β/α interfaces differ in the extent of inter-subunit interactions between the C termini of the β and α subunits. At the closed βDP/α DP interface, a hydrogen-bonding network is formed between both subunits, which is absent at the more open βTP/α TP interface and at the wide open βE/α E interface. The hydrogen-bonding network reaches from βL328 (Escherichia coli numbering) and βQ441 via αQ399, βR398, and αE402 to βR394, and ends in a cation/π interaction between βR394 and αF406. Using mutational analysis in E. coli ATP synthase, the functional importance of the βDP/αDP hydrogen-bonding network is demonstrated. Its elimination results in a severely impaired enzyme but has no pronounced effect on the binding affinities of the catalytic sites. A possible role for the hydrogen-bonding network in coupling of ATP synthesis/hydrolysis and rotation will be discussed. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.
dc.identifier.citationMao, H.Z., Abraham, C.G., Krishnakumar, A.M., & Weber, J.. 2008. A functionally important hydrogen-bonding network at the βDP/αDP interface of ATP synthase. Journal of Biological Chemistry, 283(36). https://doi.org/10.1074/jbc.M804142200
dc.identifier.urihttps://doi.org/10.1074/jbc.M804142200
dc.identifier.urihttps://hdl.handle.net/2346/95946
dc.language.isoeng
dc.titleA functionally important hydrogen-bonding network at the βDP/αDP interface of ATP synthase
dc.typeArticle

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