Impact of temperature-dependent phage expression on Pseudomonas aeruginosa biofilm formation

dc.creatorBisht, Karishma
dc.creatorMoore, Jessica L.
dc.creatorCaprioli, Richard M.
dc.creatorSkaar, Eric P.
dc.creatorWakeman, Catherine A.
dc.date.accessioned2021-09-29T20:01:34Z
dc.date.available2021-09-29T20:01:34Z
dc.date.issued2021
dc.descriptionThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_US
dc.description.abstractPseudomonas aeruginosa is a ubiquitous opportunistic pathogen that forms robust biofilms in the different niches it occupies. Numerous physiological adaptations are required as this organism shifts from soil or aquatic environments to a host-associated lifestyle. While many conditions differ between these niches, temperature shifts are a factor that can contribute to physiological stress during this transition. To understand how temperature impacts biofilm formation in this pathogen, we used proteomic and transcriptomic tools to elucidate physiological responses in environment-relevant vs. host-relevant temperatures. These studies uncovered differential expression of various proteins including a phage protein that is associated with the EPS matrix in P. aeruginosa. This filamentous phage was induced at host temperatures and was required for full biofilm-forming capacity specifically at human body temperature. These data highlight the importance of temperature shift in biofilm formation and suggest bacteriophage proteins could be a possible therapeutic target in biofilm-associated infections.en_US
dc.identifier.citationBisht, K., Moore, J.L., Caprioli, R.M. et al. Impact of temperature-dependent phage expression on Pseudomonas aeruginosa biofilm formation. npj Biofilms Microbiomes 7, 22 (2021). https://doi.org/10.1038/s41522-021-00194-8en_US
dc.identifier.urihttps://doi.org/10.1038/s41522-021-00194-8
dc.identifier.urihttps://hdl.handle.net/2346/87969
dc.language.isoengen_US
dc.subjectBiofilmsen_US
dc.subjectMicrobial Ecologyen_US
dc.subjectPathogensen_US
dc.titleImpact of temperature-dependent phage expression on Pseudomonas aeruginosa biofilm formationen_US
dc.typeArticleen_US

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