E4orf1 Prevents Progression of Fatty Liver Disease in Mice on High Fat Diet

dc.creatorAfruza, Rownock (TTU)
dc.creatorDhurandhar, Nikhil V. (TTU)
dc.creatorHegde, Vijay (TTU)
dc.date.accessioned2023-02-21T21:04:37Z
dc.date.available2023-02-21T21:04:37Z
dc.date.issued2022
dc.description© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).en_US
dc.description.abstractNon-alcoholic fatty liver disease (NAFLD) covers a broad spectrum of liver diseases ranging from steatosis to cirrhosis. There are limited data on prevention of hepatic steatosis or its progression to liver disease. Here, we tested if either transgenic (Tg) doxycycline-induced expression in adipose tissue of E4orf1 (E4), an adenoviral protein, or dietary fat restriction attenuated hepatic steatosis or its progression in mice. Twelve to fourteen-week-old TgE4 mice (E4 group) and control mice were exposed to a 60% (Kcal) high fat diet (HFD) for 20 weeks, while another group of mice on HFD for 10 weeks were switched to a chow diet (chow group) for another 10 weeks. Glycemic control was determined at weeks 10 and 20. Tissues were collected for gene and protein analysis at sacrifice. Compared to control, diet reversal significantly reduced body weight in the chow group, whereas E4 expression attenuated weight gain, despite HFD. E4 mice evinced significantly improved glucose clearance, lower endogenous insulin secretion, reduced serum triglycerides, attenuated hepatic steatosis and inflammation. Interestingly, in spite of weight loss and lower liver fat, chow mice showed significant upregulation of hepatic genes involved in lipid metabolism. Despite HFD, E4 prevents hepatic lipid accumulation and progression of hepatic steatosis, while diet reversal maintains hepatic health, but is unable to improve molecular changes.en_US
dc.identifier.citationAfruza R, Dhurandhar NV, Hegde V. E4orf1 Prevents Progression of Fatty Liver Disease in Mice on High Fat Diet. International Journal of Molecular Sciences. 2022; 23(16):9286. https://doi.org/10.3390/ijms23169286en_US
dc.identifier.urihttps://doi.org/10.3390/ijms23169286
dc.identifier.urihttps://hdl.handle.net/2346/90789
dc.language.isoengen_US
dc.subjectObesityen_US
dc.subjectNAFLDen_US
dc.subjectNASHen_US
dc.subjectE4orf1en_US
dc.subjectDiet reversalen_US
dc.titleE4orf1 Prevents Progression of Fatty Liver Disease in Mice on High Fat Dieten_US
dc.typeArticleen_US

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