Revisiting the STEC Testing Approach: Using espK and espV to Make Enterohemorrhagic Escherichia coli (EHEC) Detection More Reliable in Beef

dc.creatorDelannoy, Sabine
dc.creatorChaves, Byron D. (TTU)
dc.creatorIson, Sarah A. (TTU)
dc.creatorWebb, Hattie E. (TTU)
dc.creatorBeutin, Lothar
dc.creatorDelaval, José
dc.creatorBillet, Isabelle
dc.creatorFach, Patrick
dc.date.accessioned2023-01-27T16:57:23Z
dc.date.available2023-01-27T16:57:23Z
dc.date.issued2016
dc.description© 2016 Delannoy, Chaves, Ison, Webb, Beutin, Delaval, Billet and Fach. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.description.abstractCurrent methods for screening Enterohemorrhagic Escherichia coli (EHEC) O157 and non-O157 in beef enrichments typically rely on the molecular detection of stx, eae, and serogroup-specific wzx or wzy gene fragments. As these genetic markers can also be found in some non-EHEC strains, a number of “false positive” results are obtained. Here, we explore the suitability of five novel molecular markers, espK, espV, ureD, Z2098, and CRISPRO26:H11 as candidates for a more accurate screening of EHEC strains of greater clinical significance in industrialized countries. Of the 1739 beef enrichments tested, 180 were positive for both stx and eae genes. Ninety (50%) of these tested negative for espK, espV, ureD, and Z2098, but 12 out of these negative samples were positive for the CRISPRO26:H11 gene marker specific for a newly emerging virulent EHEC O26:H11 French clone. We show that screening for stx, eae, espK, and espV, in association with the CRISPRO26:H11 marker is a better approach to narrow down the EHEC screening step in beef enrichments. The number of potentially positive samples was reduced by 48.88% by means of this alternative strategy compared to the European and American reference methods, thus substantially improving the discriminatory power of EHEC screening systems. This approach is in line with the EFSA (European Food Safety Authority) opinion on pathogenic STEC published in 2013.en_US
dc.identifier.citationDelannoy S, Chaves BD, Ison SA, Webb HE, Beutin L, Delaval J, Billet I and Fach P (2016) Revisiting the STEC Testing Approach: Using espK and espV to Make Enterohemorrhagic Escherichia coli (EHEC) Detection More Reliable in Beef. Front. Microbiol. 7:1. doi: 10.3389/fmicb.2016.00001en_US
dc.identifier.urihttps://doi.org/10.3389/fmicb.2016.00001
dc.identifier.urihttps://hdl.handle.net/2346/90485
dc.language.isoengen_US
dc.subjectShiga Toxin-Producing Escherichia coli (STEC)en_US
dc.subjectO157en_US
dc.subjectNon-O157en_US
dc.subjectespKen_US
dc.subjectespVen_US
dc.subjectureDen_US
dc.subjectZ2098en_US
dc.subjectClustered Regularly Interspaced Short Palindromic Repeats (CRISPR)en_US
dc.titleRevisiting the STEC Testing Approach: Using espK and espV to Make Enterohemorrhagic Escherichia coli (EHEC) Detection More Reliable in Beefen_US
dc.typeArticleen_US

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