Differential Expression of miRNAs Involved in Response to Candidatus Liberibacter asiaticus Infection in Mexican Lime at Early and Late Stages of Huanglongbing Disease

dc.creatorBojórquez-Orozco, Ana Marlenne
dc.creatorArce-Leal, Ángela Paulina
dc.creatorMontes, Ricardo A.Chávez (TTU)
dc.creatorSantos-Cervantes, María Elena
dc.creatorCruz-Mendívil, Abraham
dc.creatorMéndez-Lozano, Jesús
dc.creatorCastillo, Araceli G.
dc.creatorRodríguez-Negrete, Edgar A.
dc.creatorLeyva-López, Norma Elena
dc.date.accessioned2023-04-17T21:08:54Z
dc.date.available2023-04-17T21:08:54Z
dc.date.issued2023
dc.description© 2023 by the authors. cc-by
dc.description.abstractHuanglongbing (HLB) is one of the most destructive diseases threatening citriculture worldwide. This disease has been associated with α-proteobacteria species, namely Candidatus Liberibacter. Due to the unculturable nature of the causal agent, it has been difficult to mitigate the disease, and nowadays a cure is not available. MicroRNAs (miRNAs) are key regulators of gene expression, playing an essential role in abiotic and biotic stress in plants including antibacterial responses. However, knowledge derived from non-model systems including Candidatus Liberibacter asiaticus (CLas)-citrus pathosystem remains largely unknown. In this study, small RNA profiles from Mexican lime (Citrus aurantifolia) plants infected with CLas at asymptomatic and symptomatic stages were generated by sRNA-Seq, and miRNAs were obtained with ShortStack software. A total of 46 miRNAs, including 29 known miRNAs and 17 novel miRNAs, were identified in Mexican lime. Among them, six miRNAs were deregulated in the asymptomatic stage, highlighting the up regulation of two new miRNAs. Meanwhile, eight miRNAs were differentially expressed in the symptomatic stage of the disease. The target genes of miRNAs were related to protein modification, transcription factors, and enzyme-coding genes. Our results provide new insights into miRNA-mediated regulation in C. aurantifolia in response to CLas infection. This information will be useful to understand molecular mechanisms behind the defense and pathogenesis of HLB.
dc.identifier.citationBojorquez-Orozco, A.M., Arce-Leal, A.P., Montes, R.A.C., Santos-Cervantes, M.E., Cruz-Mendivil, A., Mendez-Lozano, J., Castillo, A.G., Rodriguez-Negrete, E.A., & Leyva-Lopez, N.E.. 2023. Differential Expression of miRNAs Involved in Response to Candidatus Liberibacter asiaticus Infection in Mexican Lime at Early and Late Stages of Huanglongbing Disease. Plants, 12(5). https://doi.org/10.3390/plants12051039
dc.identifier.urihttps://doi.org/10.3390/plants12051039
dc.identifier.urihttps://hdl.handle.net/2346/92890
dc.language.isoeng
dc.subjectCandidatus Liberibacter asiaticus (CLas)
dc.subjectHuanglongbing (HLB)
dc.subjectMexican lime
dc.subjectmicroRNA
dc.subjectShortStack
dc.subjecttarget genes
dc.titleDifferential Expression of miRNAs Involved in Response to Candidatus Liberibacter asiaticus Infection in Mexican Lime at Early and Late Stages of Huanglongbing Disease
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Main article with TTU Libraries cover page.pdf
Size:
1.2 MB
Format:
Adobe Portable Document Format
Collections