Organ models are used to simulate and plan new surgical procedures or develop new tools. In recent years the demand for organ phantoms has increased substantially, especially in surgical procedures. Developing these organ phantoms requires soft and stretchy materials to mimic the human organs’ detailed morphology properly. This will enable the use of simulation tools to assess the behavior and optimize the design in the field of soft organ development. Samples from different types of synthetic biomaterials, namely Dragon Skin-10 Very fast, Dragon Skin FX-Pro, Ecoflex 00–10, Ecoflex 00–30, and Ecoflex 00–50, were subjected to uniaxial tensile tests according to the ASTM D412 standard. The samples were prepared using a dumbbell shape mold, and the uniaxial tensile test was performed using an Instron universal testing machine. The data obtained from the tensile test is further analyzed using the image analysis software ImageJ. Using the Curve Fitter 2022 tool, the curve is plotted using the Uniaxial tensile test data, and the hyperelastic model is applied to the study. In hyperelastic model analysis, it was observed that the Yeoh 3-Order Model fits into our study and the constants of the model are calculated. This study aims to provide the mechanical characterization and material properties of commercially available biomaterials and many of these materials in the field of artificial organ and soft robot development.