2024-01-172024-01-172023Macias, Franco, A., Da, Silva, A.E.M., De, Moura, F.H., Norris, A.B., Roloson, S.B., Gerrard, D.E., De, Mello, A., & Fonseca, M.A.. 2023. Effects of isoenergetic supplementation as water use mitigation strategy on water footprint and health of nursing bull calves. Translational Animal Science, 7(1). https://doi.org/10.1093/tas/txad127https://doi.org/10.1093/tas/txad127https://hdl.handle.net/2346/97500© 2023 The Author(s). Published by Oxford University Press on behalf of the American Society of Animal Science. cc-by-ncSustainable livestock systems focus on mitigating natural resource use such as water. Dietary management strategies can significantly reduce the water footprint of livestock animals; however, animal health is of concern when animals reduce water intake due to subacute dehydration. To evaluate potential consequences of this nutritional management intervention, a total of 23, 60±3 days old nursing Holstein bull calves, weighing 94.7±12.07 kg, were distributed in a completely randomized design and received one of three diets. Control was a basal diet composed of a non-medicated milk replacer (milk replacer; n=7), and the additional two diets, were composed of the same non-medicated milk replacer in addition to either lipid [n=8; milk replacer+menhaden fish oil (3 %)] or soluble carbohydrate [n=8; milk replacer+corn starch (7%) isoenergetic to fat group] supplements. Animals were offered ad libitum mineral mix and water, as well as 120 g/day of a composite mix of dried microbrewery's spent grains. Data were analyzed as linear and generalized linear mixed models with diet as a fixed effect and animal as random utilizing R studio (R Core Team, 2021, Vienna, Austria; SAS Inst., Cary, NC). Within supplementation groups, lipid supplemented calves had the highest lymphocyte (63.24 vs 57.69 counts/100 lymphocytes; P<0.033), and lowest neutrophil counts (29.3 vs 35.3 counts/100 lymphocytes; P<0.047). Supplementation significantly increased total serum protein (P=0.001) and skin moisture (P<0.011), with carbohydrate group having the highest skin moisture (5.30 vs 3.99; P<0.047). Supplementation also decreased fecal fluidity scores (P<0.001) with no significant change in serum electrolytes (P>0.256). No significant differences were found amongst treatments for the ingestive behavior (P>0.338). The carbohydrate-supplemented calves significantly decreased all daily water footprints compared to the control and fat-supplemented groups: blue a 47.55 L decrease, (P<0.001), green a 265.62 L decrease (P=0.005), and gray a 55.87 L decrease (P=0.009) water footprint, as well as total water footprint (369.04 L, P=0.004). Our results indicate the potential to maintain animal performance while increasing water use efficiency through diet supplementation tailored to mitigate water use, without adverse effects on animal health.engcalf healthcalf hydrationmetabolic waternursing calfwater footprintArticle