2022-04-072022-04-072014-052014-05May 2014https://hdl.handle.net/2346/89047Cotton fiber is the world’s most important commercial and renewable fiber. Cotton industry produces annual revenue of more than $100 billion dollars through textile, agriculture and non-woven’s. Cotton genus Gossypium has 5 allotetraploids out of which Gossypium barbadense L. account for 5% of world’s production while Gossypium hirsutum L. accounts for more than 95% of world’s production. Cotton fiber development takes place in four distinct but overlapping stages namely initiation, elongation, secondary wall biosynthesis and maturation. Properties of fiber such as finesses, strength and micronaire are determined during the early stages of fiber development. Moreover fibers produced from Gossypium barbadense have a better quality when compared to Gossypium hirsutum. In this study we analyzed the key mechanisms underlying in early elongation stages of Pima S7 (Gossypium barbadense) and TM1 (Gossypium hirsutum) using RNA Seq technology. Early elongation stages of 3 DPA and 5DPA of both these genotypes were sequenced on Illumina Mi-Seq and analyzed to obtain differentially expressed genes. The expression patterns of these genes were then validated using Real Time quantitative PCR. Furthermore four way comparison was performed between Pima 3DPA, Pima 5DPA, TM1_3DPA and TM1_5DPA and 16,656 genes were found to be differentially expressed across these two genotypes. Genes responsible for fiber development were studied from this list and belonged to 8 functional categories. Classes of genes in COBRA, hemicellulose, plasma membrane transport and sucrose degradation were highly expressed in Pima S7 when compared to TM-1.application/pdfengCotton FiberRNA Seq.Thesis2022-04-07Access is not restricted.