2016-07-282016-07-282016-07-10ICES_2016_152http://hdl.handle.net/2346/67562Puerto RicoUnited StatesUniversity of Puerto Rico, Rio Piedras CampusNew York UniversityUniversity of Puerto Rico – Rio Piedras205ICES205: Advanced Life Support Sensor and Control TechnologyVienna, AustriaNadja E. Solis-Marcano, Department of Chemistry University of Puerto Rico, Puerto RicoMarjorie Lopez-Nieves, Department of Interdisciplinary University of Puerto Rico, Puerto RicoBrismar Pinto-Pacheco, Department of Chemistry University of Puerto Rico, Puerto RicoCarlos R. Cabrera, Department of Chemistry University of Puerto Rico, Puerto RicoThe 46th International Conference on Environmental Systems was held in Vienna, Austria, USA on 10 July 2016 through 14 July 2016.Interest in miniaturized bio-sensing techniques has grown in the past decades for the rapid and accurate detection of disease-causing agents. Innovative custom microdevices provide a greener approach by reducing the cost and waste in terms of sample amount, reagent volumes, size, time and human resources. This is particularly important for outer space environment where timing is crucial and laboratory facilities are not available. Here, we propose a non-faradaic, label-free, electrochemical method based on capacitance measurement to sense DNA surface modification and hybridization. We created custom-made gold interdigital microelectrodes arrays using photolithography technique. Silver electroplating was used to make a stable silver silver/chloride quasi-reference electrode. Self assembled monolayers of B. Anthracis aptamer at two different surface coverage were made and exposed to complementary, non-complementary and mismatch strands to study the hybridization and/or non-hybridization processes by means of double layer capacitance (Cdl) measurements at two given applied potentials using Electrochemical Impedance Spectroscopy (EIS) analysis. An average percentage change in Cdl of 31.0% and 19.8% were obtained for the low and high Anthracis aptamer coverage respectively when exposed to its complementary target. The conditions that showed better distinction between strand interactions as well as lower error bars were low surface coverage at 0.3V vs. Eoc applied potential. Overall results showed that double layer capacitance is a measurable property to detect specific DNA sequences.The 46th International Conference on Environmental Systems was held in Vienna, Austria, USA on 10 July 2016 through 14 July 2016.engBiosensorCapacitanceElectrochemistryPresentation