Browsing by Author "Henry, Darren D. (TTU)"
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Item Injecting Fusobacterium necrophorum into the peripheral circulation or hepatic portal vein of preruminant Holstein calves failed to induce liver abscesses*(2024) Cox, Hannah E. (TTU); Menta, Paulo R. (TTU); Nagaraja, T. G.; Crossland, Whitney L. (TTU); Hales, Kristin E. (TTU); Henry, Darren D. (TTU); Strieder-Barboza, Clarissa (TTU); Broadway, Paul R.; Carroll, Jeffery A.; Ballou, Michael A. (TTU); Machado, Vinicius S. (TTU)Objective: Our goal was to determine whether admin- istration of Fusobacterium necrophorum ssp. necrophorum into the jugular or portal vein will induce liver abscesses in preruminant calves. Materials and Methods: The study was performed as 2 experiments according to the inoculation site: jugu- lar vein or hepatic portal circulation. Experiment 1 was performed in 18 calves randomly assigned to receive intra- jugular infusion of saline (CONIV), or 107 (FUSOIV7), 109 (FUSOIV9), and 1011 (FUSOIV11) of F. necrophorum. In experiment 2, 20 calves were assigned to receive intrapor- tal infusion of saline or 106 (FUSOPV6), 108 (FUSOPV8), and 1010 (FUSOPV10) of F. necrophorum. Blood samples were collected on d 0, 1, 3, 5, 7, and 14 for hematology. Calves were slaughtered 14 d after inoculation and exam- ined for liver gross pathology. Results and Discussion: Neither model produced liver abscesses. However, in experiment 1, inoculation increased monocyte counts in FUSOIV11 calves versus CONIV, FUSOIV7, and FUSOIV9 on d 3 and 5 postchal- lenge. The neutrophil-to-lymphocyte ratio was greater for FUSOIV11 than CONIV and FUSOIV9 on d 7 postchallenge. In experiment 2, inoculation increased monocyte and neutrophils counts in the FUSOPV8 group compared with calves in other groups. Implications and Applications: Contrary to previ- ous reports, intraportal and intrajugular inoculation with F. necrophorum did not produce liver abscesses in preru- minant calves. Further research is necessary to explore alternative methodologies to use preruminant calves in experimental disease models for liver abscesses in cattle.Item Teff grass for continuous stocking in the Southern High Plains by growing beef steers receiving protein supplements(2021) Sugg, Joel D. (TTU); Sarturi, Jhones O. (TTU); West, Charles P. (TTU); Ballou, Michael A. (TTU); Henry, Darren D. (TTU)This experiment evaluated forage quality, total nutrient yield, water footprint, and growth performance of beef steers receiving protein supplements while grazing Teff grass [‘Tiffany’Eragrostis tef (Zucc.) Trotter] over two consecutive growing seasons. Each year, four 2.66-ha irrigated paddocks (experimental units) were stocked with crossbred beef steers (n = 5 per paddock, initial BW = 289 ± 30 for yr 1; and n = 6, initial BW = 286 ± 23 for yr 2) in a randomized complete block design and stocked continuously for 63 d. Daily supplements [0.45 kg/d of cottonseed meal (Control) enough to avoid a negative ruminal N balance; and 0.50% mean paddock BW animal-daily (approximately 1.65 kg) of sorghum-dried distillers grains plus solubles, (DDGS)] were randomly assigned to two paddocks each. Supplement did not influence forage neutral detergent fiber (NDF), acid detergent fiber, crude protein, or in vitro true digestibility (P ≥ 0.54), except for a tendency (P = 0.08) for a numerical increase in NDF content of paddocks with steers that received DDGS supplementation. Paddock nutrient-yields were similar (P ≥ 0.43) between supplement treatments. Supplementation with DDGS produced greater (P = 0.01) cattle shrunk average daily gain (ADG). Predicted teff dry matter intake (DMI), net energy for maintenance (NEm), and growth (NEg) (P ≤ 0.03) were greater with cattle offered Control treatment. Predicted total DMI was similar (P = 0.14) although predicted dietary NEm, NEg, gain:feed, and total BW gain were greater (P ≤ 0.02) with DDGS. Predicted forage intake was greater (P ≤ 0.05) for cattle offered Control treatment. Teff nutrients remaining on d 56 were similar (P = 0.33) between treatments. Water footprint for total production of forage nutrient components did not differ (P ≥ 0.12) by treatments. Nutrient yield and water use efficiency of continuously stocked teff grass was not affected by supplemental regimen. Using DDGS as a supplement may increase BW gain through increased nutrient utilization without hindering teff nutrient production on a continuous stocking system.