Browsing by Author "Kahathuduwa, Chanaka N. (TTUHSC)"
Now showing 1 - 2 of 2
- Results Per Page
- Sort Options
Item Errors in the implementation, analysis, and reporting of randomization within obesity and nutrition research: a guide to their avoidance(2021) Vorland, Colby J.; Brown, Andrew W.; Dawson, John A. (TTU); Dickinson, Stephanie L.; Golzarri-Arroyo, Lilian; Hannon, Bridget A.; Heo, Moonseong; Heymsfield, Steven B.; Jaywardene, Wasantha P.; Kahathuduwa, Chanaka N. (TTUHSC); Keith, Scott W.; Oakes, J. Michael; Tekwe, Carmen D.; Thabane, Lehana; Allison, David B.Randomization is an important tool used to establish causal inferences in studies designed to further our understanding of questions related to obesity and nutrition. To take advantage of the inferences afforded by randomization, scientific standards must be upheld during the planning, execution, analysis, and reporting of such studies. We discuss ten errors in randomized experiments from real-world examples from the literature and outline best practices for their avoidance. These ten errors include: representing nonrandom allocation as random, failing to adequately conceal allocation, not accounting for changing allocation ratios, replacing subjects in nonrandom ways, failing to account for non-independence, drawing inferences by comparing statistical significance from within-group comparisons instead of between-groups, pooling data and breaking the randomized design, failing to account for missing data, failing to report sufficient information to understand study methods, and failing to frame the causal question as testing the randomized assignment per se. We hope that these examples will aid researchers, reviewers, journal editors, and other readers to endeavor to a high standard of scientific rigor in randomized experiments within obesity and nutrition research.Item Sex-Dependent Effects of Eicosapentaenoic Acid on Hepatic Steatosis in UCP1 Knockout Mice(2021) Albracht-Schulte, Kembra (TTU); Wilson, Savanna (TTU); Johnson, Paige (TTU); Pahlavani, Mandana (TTU); Ramalingam, Latha (TTU); Goonapienuwala, Bimba (TTU); Kalupahana, Nishan S. (TTU); Festuccia, William T.; Scoggin, Shane (TTU); Kahathuduwa, Chanaka N. (TTUHSC); Moustaid-Moussa, Naima (TTU)Visceral obesity may be a driving factor in nonalcoholic fatty liver disease (NAFLD) development. Previous studies have shown that the omega-3 polyunsaturated fatty acid, eicosapentaenoic acid (EPA), ameliorates obesity in high-fat (HF) fed male, C57Bl/6 mice at thermoneutral conditions, independent of uncoupling protein 1 (UCP1). Our goals herein were to investigate sex-dependent mechanisms of EPA in the livers of wild type (WT) and UCP1 knockout (KO) male and female mice fed a HF diet (45% kcal fat; WT-HF, KO-HF) with or without supplementation of 36 g/kg EPA (WT-EPA, KO-EPA). KO significantly increased body weight in males, with no significant reductions with EPA in the WT or KO groups. In females, there were no significant differences in body weight among KO groups and no effects of EPA. In males, liver TGs were significantly higher in the KO-HF group and reduced with EPA, which was not observed in females. Accordingly, gene and protein markers of mitochondrial oxidation, peroxisomal biogenesis and oxidation, as well as metabolic futile cycles were sex-dependently impacted by KO and EPA supplementation. These findings suggest a genotypic difference in response to dietary EPA supplementation on the livers of male and female mice with diet-induced obesity and housed at thermoneutrality.