Browsing by Author "Kottapalli, Kameswara Rao"
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Item Biophysical characterization of gossypium synaptotagmin-1 C2B domain(2012-05) Shanbhogue, Prajna; Sutton, Roger Bryan; Knaff, David B.; Kottapalli, Kameswara RaoSynaptotagmins constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region a variable linker, and two C-terminal C2-domains. In humans, neurotransmitter release is a process highly regulated by intracellular Ca2+ levels in association with SNARE complex assembly and SYT-1 protein. In plants SYT-1 is the first synaptotagmin protein identified to participate in Ca2+ dependent repair of membranes, thus playing an important role in stress tolerance. Our long term goal is to tune the response of Gossipium SYT-1 to abiotic stress. Understanding the quaternary structure of SYT-1 C2 in plants as well as how it interacts with its cations and phospholipid is essential to further our understanding of how it exhibits its unique properties. So far, we have been successful in; 1. Developing purification protocols for Gossipium SYT-1 C2B domain, WW and AA mutants. 2. We have circular dichroism (CD) data that shows that it predominantly contains β-sheets. 3. We have probably obtained crystals in several different conditions. 4. We have confirmed the mutants through Mass Spectrometric analysis. 5. We have shown the affinity of Gossypium SYT-1 C2B WT to cations as well as to phospholipids. 6. The effect of mutations in the Ca2+ binding loop of Gossypium SYT-1 C2B was also studied.Item Biophysical characterization of Mitsugumin 53 protein(2013-08) Nandkumar, Rohan; Sutton, Bryan R; Knaff, David B.; Kottapalli, Kameswara RaoMuscle cells undergo frequent damage and repair, which is an essential process to maintain cellular homeostasis. These processes involve plasma membrane repair and remodeling following damage. The inability of a cell to repair membrane lesions can lead to pathological conditions such as muscular dystrophy and cardiovascular diseases. Mitsugumin 53 (MG53) is a muscle-specific protein belonging to the tripartite motif (TRIM 72) family of proteins. MG53 primarily interacts with phosphatidylserine, a phospholipid that specific to the inner leaflet of the plasma membrane and cytoplasmic face of intracellular vesicles. Interactions between MG53 and dysferlin are also thought to play a role in membrane repair. This work describes the analysis of MG53 using Isothermal Titration Calorimetry (ITC), Fourier Transform Infrared (FTIR) spectroscopic analysis and Circular Dichroism (CD). These techniques are capable of measuring the thermodynamic properties that describe the interactions between MG53 and potential ligands, such as o-phosphoserine and dysferlin. The ultimate goal is to better understand the role of MG53 and its contribution to membrane repair.Item Identification of Genetic, Phenotypic, and Transcriptomic Differences in Virulence-attenuated Strains Relative to Fully Virulent Strains of Listeria monocytogenes.(2017-12-08) Cook, Peter W.; Nightingale, Kendra K.; den Bakker, Hendrik C.; Brashears, Mindy M.; Loneragan, Guy H.; Kottapalli, Kameswara RaoListeria monocytogenes is a human and animal pathogen capable of causing disease outcomes from gastroenteritis to death. In addition, it is resistant to several common food environmental stressors, and capable of growth at low temperatures. The studies presented herein attempt to differentiate strains of L. monocytogenes using genomic, phenotypic, and transcriptomic information. Chapter 1 first describes aspects of L. monocytogenes that are integral in the understanding of virulence and pathogenicity, differentiation of strains, and provides an understanding the mechanisms of evolution that may occur in the food processing environment. Chapther 2 describes the first study that used a set of virulence-attenuated strains isolated from food-associated environments that were identified with known naturally occurring premature stop codons in internalin A (inlA), an important virulence gene. Several different mutation types have been identified both across and within the lineages of L. monocytogenes. These lineages are genetically distinct suggesting the premature stop codon in inlA has arisen through either a common mechanism or has resulted from convergent mutations and could provide independent clonal complexes, or clonally related organism, with fitness increases through the loss of the primary interaction with the host. The set of strains selected were subjected to whole genome sequencing to identify any additional loss-of-function mutations, or other changes to the genome that could provide additional support for the loss of the primary host pathogenicity route. Chapter 3 describes the second study that selected two virulence-attenuated strains and two fully virulent strains from the nearest neighbor clonal complex to compare the transcriptional activity of these strains to one another. The strains were selected to represent the virulence-attenuated subpopulation or the fully virulent subpopulation based on the results of the first study including their increased biomass formation or swarming motility, having the two most common inlA PMSC types, and being isolated from food processing facilities. The transcriptomic experiment was performed by comparing each of the strains transcriptome on stainless steel to itself under planktonic conditions. The temperature, moisture, and sheer force over the coupons were included to be sure the comparisons were between planktonic and fully adherent L. monocytogenes. We were able to identify several gene categories across the clonal complexes that contained genes potentially non-functional due to premature stop codons in the whole genome sequencing data, and the transcriptomic data suggests broad differences in the number and content of differentially expressed genes between the fully virulent and virulence-attenuated strains.Item Immunological effects of yeast products on pre-weaned dairy calves(2016-05) Harris, Tyler; Ballou, Michael A.; Jackson, Samuel P.; Carroll, Jeffery A.; Trojan, Sara J.; Kottapalli, Kameswara RaoEnteric disease continues to account for a large proportion of morbidity and mortality among dairy calves in the first few weeks of life. After the first 48 hours of life until weaning, approximately 10% of calves die. There are many enteric pathogens that cause severe local and systemic disease with high risk of mortality. In the past, metaphylactic use of antibiotics was widely used in the dairy industry, but lately there has been increased concern in the metaphylactic use of antibiotics. Because of this, there is interest in finding alternatives to antibiotic use that show similar efficacy as antibiotics in reducing disease. Yeast products, specifically those derived from Saccharomyces cerevisiae, have been shown to improve performance and health characteristics in calves. The three Saccharomyces cerevisiae derived products of interest in this research are: Yeast cell wall, yeast culture, and live yeast supplements. In the first study, the effects of yeast culture were studied in Holstein dairy calves infected by Citrobacter freundii. These bacteria are opportunistic pathogens that are common in neonatal animals. Yeast culture was supplemented to calves in the milk (SmartCareTM; SC) and calf starter (XPCTM; XPC). Treatments in the study were: CON: milk replacer and calf starter with no added yeast fermentation products; SC: milk replacer with 1 g SC/calf/d and base calf starter; and SC+XPC: milk replacer with 1 g SC/calf/d and calf starter with 0.5% XPC. Calves in the SC treatment had less signs of infection, indicated by reduced neutrophil to lymphocyte ratios and lowered fecal scores after the Citrobacter freundii challenge. The SC+XPC calves had increased calf starter consumption and ADG after the Citrobacter freundii challenge, and also had reduced neutrophil to lymphocyte ratios and fecal scores after the Citrobacter freundii challenge. The results of the study indicate that calves supplemented with yeast culture had improved performance, health, and risk of scours during neonatal life. In the second study, the effects of yeast culture were studied in Holstein dairy calves infected by Salmonella enterica serotype Typhimurium. Salmonella enterica is one of the more common enteric pathogens found on dairies in the United States, and is the cause of many calf deaths. Yeast culture was supplemented to calves in the milk (SmartCareTM; SC) and calf starter (XPCTM; XPC). Treatments in the study were: CON: milk replacer and calf starter with no added yeast fermentation products; SC: milk replacer with 1 g SC/calf/d and base calf starter; and SC+XPC: milk replacer with 1 g SC/calf/d and calf starter with 0.5% XPC. The SC treatment calves had greater calf starter consumption and reduced fecal shedding of Salmonella enterica. The SC+XPC treatment calves had improved calf starter consumption, greater ADG, reduced neutrophil to lymphocyte ratio, reduced hematocrit, less hemoglobin concentration, and reduced Salmonella enterica shedding after being challenged with Salmonella enterica. These results indicate that both SC and SC+XPC improved performance and enteric health, with the combination treatment having the greatest beneficial effects. In the third and final study, the effects of supplementing milk replacer with SafmannanTM (SM) and ActiSafTM (AS) on calf health and performance throughout the pre-weaned and immediate post-weaned periods were studied. Calves were randomly assigned to treatments that included; CON: milk replacer with no added supplements; SM: milk replacer with 5g SM/calf/d; SM + AS: milk replacer with 2g SM/calf/d and 3g AS/calf/d. The SM calves had reduced leukocyte and neutrophil counts on d 10 and 28 of the study when compared to Con calves, indicating limited pathogenic bacterial exposure. The SM calves had the greatest expression of neutrophil L-selectin an also had greater percentages of neutrophils phagocytizing and producing an oxidative burst on d 28, indicating “priming” of the immune system. The SM + AS calves had increased calf starter consumption in the first weeks of the study. The least number of calves scoured throughout the study in the SM + AS treatment group. Like the SM calves, total leukocyte counts and neutrophil counts were also reduced in the SM + AS calves on d 10, when compared to Con calves. These data suggest that both yeast supplementation strategies may aid in the growth and health of pre-weaned, Holstein calves, with the SM + AS treatment having a more profound positive effect on performance and gastrointestinal health, the SM treatment having a more positive effect on neutrophil activity, and both yeast products having a positive effect on circulating white blood cell numbers.Item Metabolic benefits of annatto-extracted tocotrienol on glucose homeostasis, inflammation, and gut microbiome(2020) Chung, Eunhee; Elmassry, Moamen M. (TTU); Kottapalli, Pratibha; Kottapalli, Kameswara Rao; Kaur, Gurvinder (TTUHSC) (TTU); Dufour, Jannette M. (TTUHSC) (TTU); Wright, Kandis (TTUHSC); Ramalingam, Latha (TTUHSC) (TTU); Moustaid-Moussa, Naima (TTUHSC) (TTU); Wang, Rui (TTUHSC); Hamood, Abdul N. (TTUHSC); Shen, Chwan Li (TTUHSC) (TTU)Emerging evidence suggests that the gut microbiome plays an important role in the pathophysiology of both obesity and type 2 diabetes mellitus. We previously reported that dietary annatto-extracted tocotrienol exerts beneficial effects by modulating inflammatory responses in mice fed a high-fat diet (HFD). The purpose of this study was to test the hypothesis that tocotrienol supplementation when combined with an HFD would result in an altered gut microbiota composition. For 14 weeks, forty-eight male C57BL/6J mice were assigned to 4 groups—low-fat diet, HFD, HFD supplemented with annatto-extracted tocotrienol at 800 mg/kg diet (AT), and HFD supplemented with metformin at 200 mg/kg diet. Glucose homeostasis was assessed by glucose and insulin tolerance tests, serum and pancreas insulin levels, and histological assessments of insulin and glucagon in pancreatic tissue. The concentrations of adipokines were measured in white adipose tissues. For the gut microbiome analysis, cecal content was collected, DNA was extracted, and 16S rRNA gene sequencing was performed. AT supplementation improved glucose homeostasis and lowered resistin, leptin, and interleukin-6 levels in white adipose tissue. Relative to the HFD group, AT-supplemented mice showed a decrease in the Firmicutes to Bacteroidetes ratio and had a lower abundance of Ruminococcus lactaris, Dorea longicatena, and Lachnospiraceae family. The relative abundance of Akkermansia muciniphila was increased in the AT group compared to the low-fat diet group. The association between the metabolic improvements and the identified bacterial taxa suggests a potential metabolic modulation caused by AT supplementation through the gut microbiota composition in mice fed an HFD.Item MicroRNA regulation of gene expression under drought stress acclimation response in peanut (Arachis hypogaea L.) roots(2017-08) Kumar, Poornasree; Kottapalli, Kameswara Rao; Payton, Paxton; San Francisco, SusanDrought is one of the major devastating abiotic stress factors that affects crop productivity throughout the world. Plants tend to develop mechanisms to combat these adverse conditions. Some of these mechanisms are post transcriptionally regulated by short non-coding microRNA that directly modulate the gene expression during stress. The aim of this study was to identify the differentially expressed microRNAs and their targets having a significant role in drought stress and in acclimation in C76-16 drought tolerant peanut genotype. The mRNA and miRNA from root tissues were isolated and transcriptome profiles were generated by Illumina sequencing on HiSeq 2500 and MiSeq platforms respectively. A total of 800 million, 108bp reads representing the mRNA and 18 million, 35bp reads from miRNA sequencing were generated. A total of 3679 differentially expressed mRNA contigs and 350 differentially expressed microRNA were identified in response to drought acclimation. mRNA and miRNA expression profiles were analyzed and were found to be inversely correlated. MicroRNA-target analysis revealed that some of the drought stress and acclimation responses were mediated by these non-coding miRNAs. The microRNA target genes were mainly involved in redox reactions, transport, cell wall modifications and in transcription during drought stress and acclimation. Root transcriptome analysis revealed that auxin played a major role in lateral root formation during drought stress acclimation while drought stress responses were mediated by abscisic acid which is involved in inhibition of lateral root growth. More importantly, we found hormone signaling switch from abscisic acid (ABA) to auxin was observed during drought stress acclimation. This unique combinatorial study involving microRNA-mRNA expression will help in successful identification of molecular mechanisms that will assist in breeding improved drought tolerance in peanut.Item Molecular analysis of late-stage fiber development in upland cotton(2013-05) Sooter, Amanda; Kottapalli, Kameswara Rao; Payton, Paxton R.; San Francisco, Susan; Zabet-Moghaddam, MasoudCotton is the world's most important textile and the number one value-added crop. It plays a crucial role in the economy of Texas – supporting close to 50,000 jobs and supplying $2 billion to the state economy. Its role is even more evident in the South Plains of Texas, which supplies approximately 10% of the world's cotton. Understanding molecular events associated with the developing fiber could provide candidate targets for genetic improvement. Such modifications could lead to substantial crop enhancements, in terms of either yield or fiber quality or both, signifying considerable economic ramifications for the industry. Late-stage (21 and 24 days post-anthesis, dpa) fiber samples of the Upland cotton cultivar, TM1, were subjected to transcriptomic analysis using RNA Sequencing technology (Illumina MiSeq). Using de novo assembly in DNASTAR NGen software, the sequence reads with a quality score greater than 30 (9.2 million reads from 21 dpa and 7.5 million reads from 24 dpa) were assembled into 23942 contigs and 19750 contigs, respectively. RNA-Seq analysis using DNASTAR Array Star revealed 2928 differentially expressed transcripts. Differential expression was defined as those transcripts with 99% confidence and greater than 2.0-fold expression change between 21 and 24 dpa samples. Additionally, the proteomes from these two time-points were analyzed using nano liquid chromatography tandem mass spectrometry. The proteins were identified using a proteome created from transcriptomic data using The GPM search engine with a 1% false discovery rate. Proteins present in all replicates of each stage were annotated with the Mercator tool from Max Planck Institute. MapMan software was employed to visualize expression profiles at the pathway level of both transcriptomic and proteomic data. This mapping revealed up-regulation of pectinesterases and chromatin remodeling factors, such as DNA methyltransferases and histone deacetylases, and down-regulation of components of cell-signaling. Additionally, changes indicative of dormancy, prior to dehydration and final maturation appear to begin during this critical phase of fiber development. A complete analysis of this key transition in fiber development will be discussed.Item Molecular landscape of cotton fibers in early elongation(2013-05) Kunder, Komal Ramesh; San Francisco, Susan; Zabet-Moghaddam, Masoud; Kottapalli, Kameswara Rao; Payton, Paxton R.Cotton fibers are the dominant source of natural fibers used in the textile industry and contribute significantly to the world economy. Adverse environmental conditions negatively affect fiber characteristics, especially when the fibers are in the elongation phase of development. Improvement in the yield and quality of cotton fibers requires the identification of the molecular networks involved in fiber development. In this research, we have analyzed cotton fiber transcriptome and proteome in the early elongation phase using RNA-Seq and mass spectrometry, to identify the genes and proteins involved in different pathways altered during fiber development. TruSeq RNA sample preparation kit was used to prepare cDNA libraries from RNA extracted from Upland cotton cultivar TM-1 cotton fibers samples at 3 and 5 days post-anthesis (DPA). These libraries were sequenced using 151 bp, paired-end Illumina sequencing which produced 6,570,054 and 7,063,378 reads from 3 DPA and 5 DPA libraries, respectively. The de novo assembly of these raw reads with NGen identified 20,270 contigs in 3 DPA and 20,339 contigs in 5 DPA. The raw reads were mapped on the reference early fiber transcriptome and differential gene expression was estimated by QSeq (DNASTAR genome suite). A total of 3,177 transcripts were recognized as differentially expressed with 95% probability and at least a 2-fold change between 3 DPA and 5 DPA. These transcripts were annotated using Mercator and mapped to biological pathways using MapMan version 3.5.1. The up-regulated transcripts at 5 DPA belonged to cell-wall modification, phospholipid and sphingolipid synthesis, solute and water transporters, cytoskeletal elements and phytohormone categories; processes that were involved in cell wall extension, hinting that the fibers at this stage are involved in loosening the cell wall in anticipation of the rapid fiber elongation beyond 5 DPA. The NGen assembled transcriptomes from 6 stages of fiber development, 3 DPA, 5 DPA, 11 DPA, 17 DPA, 21 DPA and 24 DPA, were re-assembled using CAP3 assembly software to generate the cotton fiber transcriptome. We have also created a reference fiber proteome database from the fiber specific transcriptome containing 374,562 possible protein sequences for protein identification. This is the first report on the generation of a transcriptome and a proteome of the elongating cotton fibers using RNA-Seq. These databases form a significant source of information, and will contribute towards research on the improvement of cotton fiber characteristics.Item Molecular landscape of early elongating cotton fiber in two contrasting cotton species(2014-05) Namburi-Murugesan, Vignesh; Kottapalli, Kameswara Rao; Ulloa, Mauricio; Payton, PaxtonCotton fiber is the world’s most important commercial and renewable fiber. Cotton industry produces annual revenue of more than $100 billion dollars through textile, agriculture and non-woven’s. Cotton genus Gossypium has 5 allotetraploids out of which Gossypium barbadense L. account for 5% of world’s production while Gossypium hirsutum L. accounts for more than 95% of world’s production. Cotton fiber development takes place in four distinct but overlapping stages namely initiation, elongation, secondary wall biosynthesis and maturation. Properties of fiber such as finesses, strength and micronaire are determined during the early stages of fiber development. Moreover fibers produced from Gossypium barbadense have a better quality when compared to Gossypium hirsutum. In this study we analyzed the key mechanisms underlying in early elongation stages of Pima S7 (Gossypium barbadense) and TM1 (Gossypium hirsutum) using RNA Seq technology. Early elongation stages of 3 DPA and 5DPA of both these genotypes were sequenced on Illumina Mi-Seq and analyzed to obtain differentially expressed genes. The expression patterns of these genes were then validated using Real Time quantitative PCR. Furthermore four way comparison was performed between Pima 3DPA, Pima 5DPA, TM1_3DPA and TM1_5DPA and 16,656 genes were found to be differentially expressed across these two genotypes. Genes responsible for fiber development were studied from this list and belonged to 8 functional categories. Classes of genes in COBRA, hemicellulose, plasma membrane transport and sucrose degradation were highly expressed in Pima S7 when compared to TM-1.Item Molecular mechanisms underlying drought stress and acclimation responses in C76-16 Leaf (Arachis hypogaea L.)(2017-08) Challa, Ashwinireddy; Kottapalli, Kameswara Rao; San Francisco, Susan; Payton, Paxton R.Peanut is the third most important oilseed and legume crop in the world known for its high protein content. It is an excellent food source to overcome malnutrition in developing and under developed countries. More than 80% of the world peanut production is from arid and semi-arid regions where water is a limiting factor. Plants adapt to these stress conditions by undergoing various physiological, morphological, and molecular changes. To develop peanut drought tolerant lines, understanding drought stress and acclimation responses is very important. In this study, real field conditions were mimicked in a greenhouse experiment using C76-16, a national drought tolerant peanut check variety. A 108 bp paired end RNA-Seq was performed on 24 leaf samples from three time points. Gene expression analysis between acclimated and control, non-acclimated and control at second dry down resulted in 6916 and 8937 differentially expressed contigs. Downstream analysis using Mercator and MapMan revealed that there are no significant changes between the acclimated and the non-acclimated plants during drought stress in C76-16 genotype. In addition, drought tolerance responses in C76-16 are mainly regulated by transcription factors that act as positive and negative regulators of drought stress tolerance. The drought stress specific transcription factors identified in this study are MYB44, bHLH122, NF-YA5, MYB20, MYB60, NLP7, which are involved in regulating stomatal movements. DREB, HSF-A2 and bHLH112 were also identified which play an important role in drought tolerance. In addition, accumulation of stress related molecules such as proline, glycine betaine, H+-ATPase, aquaporins, and LEA proteins were found to play a prominent role in drought tolerance. Ubiquitination related proteins such as XERICO helps in ABA homeostasis which is important in conferring drought tolerance. Overall, this study provides an overview of drought tolerant mechanisms in the leaves of C76-16 which can contribute in breeding drought tolerant peanut genotypes.Item Molecular mechanisms underlying sugarcane response to aluminum stress by rna-seq(2020) Rosa-Santos, Thiago Mateus; da Silva, Renan Gonçalves; Kumar, Poornasree (TTU); Kottapalli, Pratibha; Crasto, Chiquito (TTU); Kottapalli, Kameswara Rao; França, Suzelei Castro; Zingaretti, Sonia MarliSome metals are beneficial to plants and contribute to critical physiological processes. Some metals, however, are not. The presence of aluminum ions (Al3+ ) can be very toxic, especially in acidic soils. Considerable parts of the world’s arable land are acidic in nature; mechanistically elucidating a plant’s response to aluminum stress is critical to mitigating this stress and improving the quality of plants. To identify the genes involved in sugarcane response to aluminum stress, we generated 372 million paired-end RNA sequencing reads from the roots of CTC-2 and RB855453, which are two contrasting cultivars. Data normalization resulted in 162,161 contigs (contiguous sequences) and 97,335 genes from a de novo transcriptome assembly (trinity genes). A total of 4858 and 1307 differently expressed genes (DEGs) for treatment versus control were identified for the CTC-2 and RB855453 cultivars, respectively. The DEGs were annotated into 34 functional categories. The majority of the genes were upregulated in the CTC-2 (tolerant cultivar) and downregulated in RB855453 (sensitive cultivar). Here, we present the first root transcriptome of sugarcane under aluminum stress. The results and conclusions of this study are a crucial launch pad for future genetic and genomic studies of sugarcane. The transcriptome analysis shows that sugarcane tolerance to aluminum may be explained by an efficient detoxification mechanism combined with lateral root formation and activation of redox enzymes. We also present a hypothetical model for aluminum tolerance in the CTC-2 cultivar.Item Targeting obesity-related inflammation through nutritional and genetic approaches(2015-08) LeMieux, Monique J.; Moustaid-Moussa, Naima; Wang, Shu; Collie, Nathan L.; Rahman, Shaikh Mizanoor; Kottapalli, Kameswara RaoOver one-third of adults in America suffer from obesity, a disease that is associated with the over-expansion of adipose tissue, along with increases in blood pressure, glycemia, inflammation and thrombosis. Low-grade chronic inflammation has been causally linked with obesity, though the mechanisms involved are not clear. Therefore for this dissertation, we were interested in developing genetic and nutritional approaches that could be used to target obesity-induced inflammation and dissect potential mechanisms involved. Specifically, we determined the role of the renin-angiotensin system (RAS) and the protective benefits of the omega-3 polyunsaturated fatty acid, eicosapentaenoic acid (EPA), on adipose tissue dysfunction and inflammation. We demonstrated in this work that inactivation of angiotensinogen (Agt) specifically in adipose tissue reduced features of obesity-induced inflammation including total macrophage infiltration, and pro-inflammatory cytokine production while increasing metabolic activity. We also showed that EPA ameliorated high-fat diet effects at least in part by increasing oxygen consumption and fatty acid oxidation, reducing adipocyte size, adipogenesis and adipose tissue inflammation, independent of obesity. In conclusion, our findings indicate that EPA feeding and inactivation of Agt could be used as a potential means to reduce inflammation locally in adipose tissue. Both approaches provided insight into potential mechanisms involved in obesity-associated insulin resistance and inflammation and may prove valuable for future clinical studies using nutritional/ pharmacological therapies against obesity and other inflammatory metabolic disorders.Item Transcriptomics of drought stress acclimation response in peanut (Arachis hypogaea L.)(2015-05) Arun, Sandhiya; Kottapalli, Kameswara Rao; Payton, PaxtonPeanut (Arachis hypogaea L.) is the second most important legume in the world and provides inexpensive but significant source of protein and edible oils.However, more than half of the peanut production area falls under arid and semi-arid regions, where peanuts are frequently subjected to drought stresses for different duration and intensities. In the United States, peanut is produced widely in the Texas High Plains region but the declining levels of water in the Ogallala aquifer has limited the available irrigation capacity to these regions. In a typical center-pivot irrigation system, plants undergo repeated cycles of water-deficit and recovery. Hence, there is a need to cultivate stress-tolerant peanut varieties that can adapt to these altering water-deficit cycles. In order to do so, first the acclimation response has to be well studied. In this study, a relatively tolerant peanut genotype from the US mini core collection was subjected to water-deficit stress acclimation. RNA-Seq was performed on root tissues using 108 bp paired end sequencing on an Illumina HiSeq2500 sequencer. Pairwise comparisons between different time points identified 13,655 differentially expressed genes(DEGs).Functional annotation of DEGs revealed that acclimation was controlled by several interacting pathways like calcium and G-protein coupled receptor mediated signaling, regulation by WRKY and R2R3-MYB transcription factors, proline accumulation, and activity of oxygen scavengers. The phytohormone ABA acted as a central mediator in generating this root-to-shoot stress response. Interestingly, we found that methionine accumulation is important for drought-stress acclimation in peanut. Some of these genes could be utilized by peanut breeders to improve water-deficit stress acclimation in field conditions.