Browsing by Author "Strieder-Barboza, Clarissa (TTU)"
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Item Injecting Fusobacterium necrophorum into the peripheral circulation or hepatic portal vein of preruminant Holstein calves failed to induce liver abscesses*(2024) Cox, Hannah E. (TTU); Menta, Paulo R. (TTU); Nagaraja, T. G.; Crossland, Whitney L. (TTU); Hales, Kristin E. (TTU); Henry, Darren D. (TTU); Strieder-Barboza, Clarissa (TTU); Broadway, Paul R.; Carroll, Jeffery A.; Ballou, Michael A. (TTU); Machado, Vinicius S. (TTU)Objective: Our goal was to determine whether admin- istration of Fusobacterium necrophorum ssp. necrophorum into the jugular or portal vein will induce liver abscesses in preruminant calves. Materials and Methods: The study was performed as 2 experiments according to the inoculation site: jugu- lar vein or hepatic portal circulation. Experiment 1 was performed in 18 calves randomly assigned to receive intra- jugular infusion of saline (CONIV), or 107 (FUSOIV7), 109 (FUSOIV9), and 1011 (FUSOIV11) of F. necrophorum. In experiment 2, 20 calves were assigned to receive intrapor- tal infusion of saline or 106 (FUSOPV6), 108 (FUSOPV8), and 1010 (FUSOPV10) of F. necrophorum. Blood samples were collected on d 0, 1, 3, 5, 7, and 14 for hematology. Calves were slaughtered 14 d after inoculation and exam- ined for liver gross pathology. Results and Discussion: Neither model produced liver abscesses. However, in experiment 1, inoculation increased monocyte counts in FUSOIV11 calves versus CONIV, FUSOIV7, and FUSOIV9 on d 3 and 5 postchal- lenge. The neutrophil-to-lymphocyte ratio was greater for FUSOIV11 than CONIV and FUSOIV9 on d 7 postchallenge. In experiment 2, inoculation increased monocyte and neutrophils counts in the FUSOPV8 group compared with calves in other groups. Implications and Applications: Contrary to previ- ous reports, intraportal and intrajugular inoculation with F. necrophorum did not produce liver abscesses in preru- minant calves. Further research is necessary to explore alternative methodologies to use preruminant calves in experimental disease models for liver abscesses in cattle.Item Lumican modulates adipocyte function in obesity-associated type 2 diabetes(2022) Strieder-Barboza, Clarissa (TTU); Flesher, Carmen G; Geletka, Lynn M; Eichler, Tad; Akinleye, Olukemi; Ky, Alexander; Ehlers, Anne P; Lumeng, Carey N; O’Rourke, Robert WObesity-associated type 2 diabetes (DM) leads to adipose tissue dysfunction. Lumican is a proteoglycan implicated in obesity, insulin resistance (IR), and adipocyte dysfunction. Using human visceral adipose tissue (VAT) from subjects with and without DM, we studied lumican effects on adipocyte function. Lumican was increased in VAT and adipocytes in DM. Lumican knockdown in adipocytes decreased lipolysis and improved adipogenesis and insulin sensitivity in VAT adipocytes in DM, while treatment with human recombinant lumican increased lipolysis and impaired insulin-sensitivity in an ERK-dependent manner. We demonstrate that lumican impairs adipocyte metabolism, partially via ERK signalling, and is a potential target for developing adipose tissue-targeted therapeutics in DM.Item Single-nuclei analysis reveals depot-specific transcriptional heterogeneity and depot-specific cell types in adipose tissue of dairy cows(2022) Michelotti, Tainara C. (TTU); Kisby, Brent R.; Flores, Lauryn S. (TTU); Tegeler, Alexandra P. (TTU); Fokar, Mohamed (TTU); Crasto, Chiquito (TTU); Menarim, Bruno C.; Loux, Shavahn C.; Strieder-Barboza, Clarissa (TTU)Adipose tissue (AT) is an endocrine organ with a central role on whole-body energy metabolism and development of metabolic diseases. Single-cell and single-nuclei RNA sequencing (scRNA-seq and snRNA-seq, respectively) analyses in mice and human AT have revealed vast cell heterogeneity and functionally distinct subtypes that are potential therapeutic targets to metabolic disease. In periparturient dairy cows, AT goes through intensive remodeling and its dysfunction is associated with metabolic disease pathogenesis and decreased productive performance. The contributions of depot-specific cells and subtypes to the development of diseases in dairy cows remain to be studied. Our objective was to elucidate differences in cellular diversity of visceral (VAT) and subcutaneous (SAT) AT in dairy cows at the single-nuclei level. We collected matched SAT and VAT samples from three dairy cows and performed snRNA-seq analysis. We identified distinct cell types including four major mature adipocytes (AD) and three stem and progenitor cells (ASPC) subtypes, along with endothelial cells (EC), mesothelial cells (ME), immune cells, and pericytes and smooth muscle cells. All major cell types were present in both SAT and VAT, although a strong VAT-specificity was observed for ME, which were basically absent in SAT. One ASPC subtype was defined as adipogenic (PPARG+) while the other two had a fibro-adipogenic profile (PDGFRA+). We identified vascular and lymphatic EC subtypes, and different immune cell types and subtypes in both SAT and VAT, i.e., macrophages, monocytes, T cells, and natural killer cells. Not only did VAT show a greater proportion of immune cells, but these visceral immune cells had greater activation of pathways related to immune and inflammatory response, and complement cascade in comparison with SAT. There was a substantial contrast between depots for gene expression of complement cascade, which were greatly expressed by VAT cell subtypes compared to SAT, indicating a pro-inflammatory profile in VAT. Unprecedently, our study demonstrated cell-type and depot-specific heterogeneity in VAT and SAT of dairy cows. A better understanding of depot-specific molecular and cellular features of SAT and VAT will aid in the development of AT-targeted strategies to prevent and treat metabolic disease in dairy cows, especially during the periparturient period.