Browsing by Author "Tang, Lili"
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Item In vitro evaluation of the cytotoxic potential of Brickellia cavanillesi (Asteraceae) using HepG2 cells(Texas Tech University, 2007-05) Viñas, René; Smith, Ernest E.; Tang, Lili; Gao, WeiminCharacterization of the potential toxicity and biological mechanisms of alternative herbal medicine offer significant benefits for people using them to treat chronic diseases or symptoms. The Latin American tea "prodigiosa" (Brickellia cavanillesi), a member of the Asteraceae botanical family is traditionally consumed as an herbal remedy to treat diabetes and other chronic diseases in several underdeveloped countries. However, there is limited data on the potential toxicity or mechanism of action for B. cavanillesi. Consequently, in this study we used an in vitro cell culture approach to evaluate cellular toxicity of an aqueous extract of B. cavanillesi. Hepatocellular carcinoma cells (HepG2) were submitted to both a dose- (IC50) and a time-dependent toxicity evaluation in the presence or absence of Fetal Bovine Serum (FBS). Herbal extracts (1-35%) were used for the IC50 study. In the time course study, cells were exposed to 1-10% extract solutions of B. cavanillesi for 48hrs. Cell viability was determined with the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. No significant differences were observed when cells were cultured with or without FBS. In this study the IC50 ranged from 5-10% in the absence and presence of FBS. At doses exceeding 10%, cellular viability decreased significantly (pItem Mitigation of Oxidative Damage by Green Tea Polyphenols and Tai Chi Exercise in Postmenopausal Women with Osteopenia(2012) Qian, Guoqing; Xue, Kathy; Tang, Lili; Wang, Franklin; Song, Xiao; Chyu, Ming Chien (TTU); Pence, Barbara C. (TTUHSC); Shen, Chwan Li (TTUHSC) (TTUHSC) (TTUHSC); Wang, Jia ShengBackground: Osteoporosis is a degenerative bone disease predominantly in postmenopausal women. Green tea polyphenols (GTP) and Tai Chi (TC) have been shown to be beneficial on human bone health. This study examined the efficacy of GTP and TC on mitigation of oxidative damage in postmenopausal women with osteopenia. Methods: A 6-month randomized and placebo-controlled clinical trial was conducted in 171 postmenopausal women with osteopenia, who were recruited from Lubbock County, Texas. These participants were treated with placebo, GTP (500 mg daily), placebo + TC (60-minute group exercise, 3 times/week), or GTP (500 mg daily) + TC (60-minute group exercise, 3 times/week), respectively. Their blood and urine samples were collected at the baseline, 1-, 3- and 6-months during intervention for assessing levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG), an oxidative DNA damage biomarker, and concentrations of serum and urine GTP components. Results: The elevated concentrations of serum and urinary GTP components demonstrated a good adherence for the trial. A significant reduction of urinary 8-OHdG concentrations was found in all three treated groups during 3-month (P<0.001) and 6-month (P<0.001) intervention, as compared to the placebo group. The significant time- and dose-effects on mitigation of the oxidative damage biomarker were also found for GTP, TC, and GTP+TC intervened groups. Conclusion: Our study demonstrated that GTP and TC interventions were effective strategies of reducing the levels of oxidative stress, a putative mechanism for osteoporosis in postmenopausal women, and more importantly, working in an additive manner, which holds the potential as alternative tools to improve bone health in this population. Trial Registration: ClinicalTrials.gov NCT00625391. © 2012 Qian et al.