The Arabidopsis RNA Binding Protein with K Homology Motifs, SHINY1, Interacts with the C-terminal Domain Phosphatase-like 1 (CPL1) to Repress Stress-Inducible Gene Expression

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dc.creatorJiang, Jiafu
dc.creatorWang, Bangshing
dc.creatorShen, Yun
dc.creatorWang, Hui
dc.creatorFeng, Qing
dc.creatorShi, Huazhong
dc.date.accessioned2021-11-18T19:43:03Z
dc.date.available2021-11-18T19:43:03Z
dc.date.issued2013
dc.description© 2013 Jiang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.description.abstractThe phosphorylation state of the C-terminal domain (CTD) of the RNA polymerase II plays crucial roles in transcription and mRNA processing. Previous studies showed that the plant CTD phosphatase-like 1 (CPL1) dephosphorylates Ser-5-specific CTD and regulates abiotic stress response in Arabidopsis. Here, we report the identification of a K-homology domain-containing protein named SHINY1 (SHI1) that interacts with CPL1 to modulate gene expression. The shi1 mutant was isolated from a forward genetic screening for mutants showing elevated expression of the luciferase reporter gene driven by a salt-inducible promoter. The shi1 mutant is more sensitive to cold treatment during vegetative growth and insensitive to abscisic acid in seed germination, resembling the phenotypes of shi4 that is allelic to the cpl1 mutant. Both SHI1 and SHI4/CPL1 are nuclear-localized proteins. SHI1 interacts with SHI4/CPL1 in vitro and in vivo. Loss-of-function mutations in shi1 and shi4 resulted in similar changes in the expression of some stress-inducible genes. Moreover, both shi1 and shi4 mutants display higher mRNA capping efficiency and altered polyadenylation site selection for some of the stress-inducible genes, when compared with wild type. We propose that the SHI1-SHI4/CPL1 complex inhibits transcription by preventing mRNA capping and transition from transcription initiation to elongation.en_US
dc.identifier.citationJiang J, Wang B, Shen Y, Wang H, Feng Q, et al. (2013) The Arabidopsis RNA Binding Protein with K Homology Motifs, SHINY1, Interacts with the Cterminal Domain Phosphatase-like 1 (CPL1) to Repress Stress-Inducible Gene Expression. PLoS Genet 9(7): e1003625. doi:10.1371/journal.pgen.1003625en_US
dc.identifier.urihttps://doi.org/10.1371/journal.pgen.1003625
dc.identifier.urihttps://hdl.handle.net/2346/88302
dc.language.isoengen_US
dc.subjectMessenger RNAen_US
dc.subjectGene Expressionen_US
dc.subjectPolyadenylationen_US
dc.subjectArabidopsis Thalianaen_US
dc.subjectProtein Domainsen_US
dc.subjectLuciferaseen_US
dc.subjectGene Regulationen_US
dc.subjectGenetic Screensen_US
dc.titleThe Arabidopsis RNA Binding Protein with K Homology Motifs, SHINY1, Interacts with the C-terminal Domain Phosphatase-like 1 (CPL1) to Repress Stress-Inducible Gene Expressionen_US
dc.typeArticleen_US

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