Anti-Inflammatory Mechanisms of Curcumin and Its Metabolites in White Adipose Tissue and Cultured Adipocytes

Simple item page
dc.creatorIslam, Tariful (TTU)
dc.creatorScoggin, Shane (TTU)
dc.creatorGong, Xiaoxia (TTU)
dc.creatorZabet-Moghaddam, Masoud (TTU)
dc.creatorKalupahana, Nishan S. (TTU)
dc.creatorMoustaid-Moussa, Naima (TTU)
dc.date.accessioned2024-02-21T21:22:23Z
dc.date.available2024-02-21T21:22:23Z
dc.date.issued2024
dc.description© 2023 by the authors. cc-by
dc.description.abstractThe plant-derived polyphenol curcumin alleviates the inflammatory and metabolic effects of obesity, in part, by reducing adipose tissue inflammation. We hypothesized that the benefits of curcumin supplementation on diet-induced obesity and systemic inflammation in mice occur through downregulation of white adipose tissue (WAT) inflammation. The hypothesis was tested in adipose tissue from high-fat diet-induced obese mice supplemented with or without curcumin and in 3T3-L1 adipocytes treated with or without curcumin. Male B6 mice were fed a high-fat diet (HFD, 45% kcal fat) with or without 0.4% (w/w) curcumin supplementation (HFC). Metabolic changes in these mice have been previously reported. Here, we determined the serum levels of the curcumin metabolites tetrahydrocurcumin (THC) and curcumin-O-glucuronide (COG) using mass spectrometry. Moreover, we determined interleukin 6 (IL-6) levels and proteomic changes in LPS-stimulated 3T3-L1 adipocytes treated with or without curcumin by using immunoassays and mass spectrometry, respectively, to gain further insight into any altered processes. We detected both curcumin metabolites, THC and COG, in serum samples from the curcumin-fed mice. Both curcumin and its metabolites reduced LPS-induced adipocyte IL-6 secretion and mRNA levels. Proteomic analyses indicated that curcumin upregulated EIF2 and mTOR signaling pathways. Overall, curcumin exerted anti-inflammatory effects in adipocytes, in part by reducing IL-6, and these effects may be linked to the upregulation of the mTOR signaling pathway, warranting additional mechanistic studies on the effects of curcumin and its metabolites on metabolic health.
dc.identifier.citationIslam, T., Scoggin, S., Gong, X., Zabet-Moghaddam, M., Kalupahana, N.S., & Moustaid-Moussa, N.. 2024. Anti-Inflammatory Mechanisms of Curcumin and Its Metabolites in White Adipose Tissue and Cultured Adipocytes. Nutrients, 16(1). https://doi.org/10.3390/nu16010070
dc.identifier.urihttps://doi.org/10.3390/nu16010070
dc.identifier.urihttps://hdl.handle.net/2346/97626
dc.language.isoeng
dc.subject3T3-L1 adipocytes
dc.subjectC57BL/6J (B6) mice
dc.subjectcurcumin
dc.subjectcurcumin-O-glucuronide
dc.subjecthigh-fat diet
dc.subjectinflammation
dc.subjectobesity
dc.subjecttetrahydrocurcumin
dc.subjectwhite adipose tissue
dc.titleAnti-Inflammatory Mechanisms of Curcumin and Its Metabolites in White Adipose Tissue and Cultured Adipocytes
dc.typeArticle

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Main article with TTU Libraries cover page.pdf
Size:
2.13 MB
Format:
Adobe Portable Document Format
Download

Collections

Faculty Research