The Golgi‑localized sphingosine‑1‑phosphate phosphatase is indispensable for Leishmania major

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dc.creatorOkundaye, Brian (TTU)
dc.creatorBiyani, Neha (TTU)
dc.creatorMoitra, Samrat (TTU)
dc.creatorZhang, Kai (TTU)
dc.date.accessioned2023-03-15T17:12:10Z
dc.date.available2023-03-15T17:12:10Z
dc.date.issued2022
dc.descriptionOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.en_US
dc.description.abstractSphingosine-1-phosphate phosphatase (SPP) catalyzes the dephosphorylation of sphingosine-1-phosphate (S1P) into sphingosine, the reverse reaction of sphingosine kinase. In mammals, S1P acts as a potent bioactive molecule regulating cell proliferation, migration, and immunity. In Leishmania, S1P production is crucial for the synthesis of ethanolamine and choline phospholipids, and cell survival under stress conditions. To better understand the roles of S1P, we characterized a SPP ortholog in Leishmania major which displays activity towards S1P but not structurally related lipids such as ceramide-1-phosphate or lysophosphatidic acid. While this enzyme is found in the endoplasmic reticulum in mammalian cells, L. major SPP is localized at the Golgi apparatus. Importantly, chromosomal SPP alleles cannot be deleted from L. major even with the addition of a complementing episome, suggesting that endogenously expressed SPP is essential. Finally, SPP overexpression in L. major leads to a slower growth rate and heightened sensitivity to brefeldin A and sodium orthovanadate. Together, these results suggest that the equilibrium between S1P and sphingosine is vital for the function of Golgi apparatus in Leishmania.en_US
dc.identifier.citationOkundaye, B., Biyani, N., Moitra, S. et al. The Golgi-localized sphingosine-1-phosphate phosphatase is indispensable for Leishmania major. Sci Rep 12, 16064 (2022). https://doi.org/10.1038/s41598-022-20249-wen_US
dc.identifier.urihttps://doi.org/10.1038/s41598-022-20249-w
dc.identifier.urihttps://hdl.handle.net/2346/91850
dc.language.isoengen_US
dc.subjectLeishmaniaen_US
dc.subjectGolgi apparatusen_US
dc.subjectcell survivalen_US
dc.subjectlipid metabolismen_US
dc.subjectsphingosineen_US
dc.subjectceramideen_US
dc.subjectsandfliesen_US
dc.titleThe Golgi‑localized sphingosine‑1‑phosphate phosphatase is indispensable for Leishmania majoren_US
dc.typeArticleen_US

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