Capacitive Biosensing Technique for the Detection of DNA Modification and Hybridization Process Using Tailored Interdigital Microelectrode Arrays

Simple item page
dc.creatorSolis-Marcano, Nadja E.
dc.creatorLopez-Nieves, Marjorie
dc.creatorPinto-Pacheco, Brismar
dc.creatorCabrera, Carlos R.
dc.date.accessioned2016-07-28T18:24:00Z
dc.date.available2016-07-28T18:24:00Z
dc.date.issued2016-07-10
dc.descriptionPuerto Rico
dc.descriptionUnited States
dc.descriptionUniversity of Puerto Rico, Rio Piedras Campus
dc.descriptionNew York University
dc.descriptionUniversity of Puerto Rico – Rio Piedras
dc.description205
dc.descriptionICES205: Advanced Life Support Sensor and Control Technology
dc.descriptionVienna, Austria
dc.descriptionNadja E. Solis-Marcano, Department of Chemistry University of Puerto Rico, Puerto Rico
dc.descriptionMarjorie Lopez-Nieves, Department of Interdisciplinary University of Puerto Rico, Puerto Rico
dc.descriptionBrismar Pinto-Pacheco, Department of Chemistry University of Puerto Rico, Puerto Rico
dc.descriptionCarlos R. Cabrera, Department of Chemistry University of Puerto Rico, Puerto Rico
dc.descriptionThe 46th International Conference on Environmental Systems was held in Vienna, Austria, USA on 10 July 2016 through 14 July 2016.
dc.description.abstractInterest in miniaturized bio-sensing techniques has grown in the past decades for the rapid and accurate detection of disease-causing agents. Innovative custom microdevices provide a greener approach by reducing the cost and waste in terms of sample amount, reagent volumes, size, time and human resources. This is particularly important for outer space environment where timing is crucial and laboratory facilities are not available. Here, we propose a non-faradaic, label-free, electrochemical method based on capacitance measurement to sense DNA surface modification and hybridization. We created custom-made gold interdigital microelectrodes arrays using photolithography technique. Silver electroplating was used to make a stable silver silver/chloride quasi-reference electrode. Self assembled monolayers of B. Anthracis aptamer at two different surface coverage were made and exposed to complementary, non-complementary and mismatch strands to study the hybridization and/or non-hybridization processes by means of double layer capacitance (Cdl) measurements at two given applied potentials using Electrochemical Impedance Spectroscopy (EIS) analysis. An average percentage change in Cdl of 31.0% and 19.8% were obtained for the low and high Anthracis aptamer coverage respectively when exposed to its complementary target. The conditions that showed better distinction between strand interactions as well as lower error bars were low surface coverage at 0.3V vs. Eoc applied potential. Overall results showed that double layer capacitance is a measurable property to detect specific DNA sequences.
dc.format.mimetypeThe 46th International Conference on Environmental Systems was held in Vienna, Austria, USA on 10 July 2016 through 14 July 2016.
dc.identifier.otherICES_2016_152
dc.identifier.urihttp://hdl.handle.net/2346/67562
dc.language.isoeng
dc.publisher46th International Conference on Environmental Systems
dc.subjectBiosensor
dc.subjectCapacitance
dc.subjectElectrochemistry
dc.titleCapacitive Biosensing Technique for the Detection of DNA Modification and Hybridization Process Using Tailored Interdigital Microelectrode Arrays
dc.typePresentation

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
ICES_2016_152.pdf
Size:
1.67 MB
Format:
Adobe Portable Document Format
Download

Collections

International Conference on Environmental Systems