Characterization of Microbes Present in Purge Pump and Separator Assembly Ground Testing

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dc.creatorSaban, Kristen
dc.creatorHerrneckar, Peyton
dc.creatorVelez Justiniano, Yo-Ann
dc.creatorLong, David
dc.creatorBeitle, Eric R.
dc.date.accessioned2023-06-15T16:13:31Z
dc.date.available2023-06-15T16:13:31Z
dc.date.issued2023-07-16
dc.descriptionKristen Saban, NASA Marshall Space Flight Center, USA
dc.descriptionPeyton Herrneckar, NASA Marshall Space Flight Center, USA
dc.descriptionYo-Ann Velez Justiniano, NASA Marshall Space Flight Center, USA
dc.descriptionDavid Long, NASA Marshall Space Flight Center, USA
dc.descriptionEric R. Beitle, NASA Marshall Space Flight Center, USA
dc.descriptionICES303: Physio-Chemical Life Support- Water Recovery & Management Systems- Technology and Process Development
dc.descriptionThe 52nd International Conference on Environmental Systems was held in Calgary, Canada, on 16 July 2023 through 20 July 2023.
dc.description.abstractMicrobiology is a growing sector in the field of human spaceflight development. Microorganisms have been observed and studied on crewed missions and space stations. The presence of microbes has potentially detrimental effects on crew health and hardware structure and maintenance. These effects are especially present on flight hardware involved with human wastewater, such as the Water Recovery System (WRS) of the International Space Station (ISS). The Urine Processor Assembly (UPA) of the WRS was recently updated to include the Purge Pump and Separator Assembly (PPSA). The PPSA improves the purge capability and therefore overall performance of the UPA. This paper details microorganisms present within three PPSA pumps after ground testing. Ground microbial testing and decontamination are essential for preventing further system contamination during flight technology demonstrations. The microbial characterization from these pumps includes qualitative and quantitative descriptions of bacterial and fungal species present. DNA sequencing, genome assembly, and identification were performed using Nanopore MinION and KBase. All three sampled pumps showed microbial growth. Bacteria were identified in both the Burkholderia and Ralstonia genera. This work has implications for the viability of urine processor technology against biofouling for longer duration spaceflight missions where hardware replacement is infeasible.
dc.identifier.otherICES-2023-87
dc.identifier.urihttps://hdl.handle.net/2346/94545
dc.language.isoeng
dc.publisher2023 International Conference on Environmental Systems
dc.subjectpurge
dc.subjectpump
dc.subjectassembly
dc.subjectECLSS
dc.subjectLSS
dc.subjectNASA
dc.subjectwater
dc.subjectUrine Processor Assembly
dc.subjectPurge Pump and Separator Assembly
dc.titleCharacterization of Microbes Present in Purge Pump and Separator Assembly Ground Testingen_US
dc.typePresentations

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