The role of promoter cis-element, mRNA capping, and ROS in the repression and salt-inducible expression of AtSOT12 in Arabidopsis

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dc.creatorChen, Jinhua
dc.creatorWang, Bangshing
dc.creatorChung, Jung-Sung
dc.creatorChai, Haoxi
dc.creatorLiu, Chunlin
dc.creatorRuan, Ying
dc.creatorShi, Huazhong
dc.date.accessioned2021-11-16T19:26:59Z
dc.date.available2021-11-16T19:26:59Z
dc.date.issued2015
dc.descriptionCopyright © 2015 Chen, Wang, Chung, Chai, Liu, Ruan and Shi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.description.abstractInducible gene expression is a gene regulatory mechanism central to plant response to environmental cues. The inducible genes are often repressed under normal growth conditions while their expression levels are significantly elevated by conditions such as abiotic stresses. Induction of gene expression requires both cis-acting DNA elements and trans-acting proteins that are modulated through signal transduction pathways. Here we report several molecular events that affect salt induced expression of the Arabidopsis AtSOT12 gene. Promoter deletion analysis revealed that DNA elements residing in the 5′ UTR are required for the salt induced expression of AtSOT12. Cytosine methylation in the promoter was low and salt stress slightly increased the DNA methylation level, suggesting that DNA methylation may not contribute to AtSOT12 gene repression. Co-transcriptional processing of AtSOT12 mRNA including capping and polyadenylation site selection was also affected by salt stress. The percentage of capped mRNA increased by salt treatment, and the polyadenylation sites were significantly different before and after exposure to salt stress. The expression level of AtSOT12 under normal growth conditions was markedly higher in the oxi1 mutant defective of reactive oxygen species (ROS) signaling than in the wild type. Moreover, AtSOT12 transcript level was elevated by treatments with DPI and DMTU, two chemicals preventing ROS accumulation. These results suggest that repression of AtSOT12 expression may require physiological level of ROS and ROS signaling.en_US
dc.identifier.citationChen J, Wang B, Chung J-S, Chai H, Liu C, Ruan Y and Shi H (2015) The role of promoter cis-element, mRNA capping, and ROS in the repression and salt-inducible expression of AtSOT12 in Arabidopsis. Front. Plant Sci. 6:974. doi: 10.3389/fpls.2015.00974en_US
dc.identifier.urihttps://doi.org/10.3389/fpls.2015.00974
dc.identifier.urihttps://hdl.handle.net/2346/88293
dc.language.isoengen_US
dc.subjectAtSOT12en_US
dc.subjectSalt Stressen_US
dc.subjectGene Regulationen_US
dc.subjectROSen_US
dc.subjectPromoter Analysisen_US
dc.subjectmRNA Cappingen_US
dc.subjectPolyadenylationen_US
dc.titleThe role of promoter cis-element, mRNA capping, and ROS in the repression and salt-inducible expression of AtSOT12 in Arabidopsisen_US
dc.typeArticleen_US

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