Exploration into the developmental toxicity of organophosphate insecticides in zebrafish (Danio rerio)

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dc.contributor.committeeChairCrago, Jordan
dc.contributor.committeeMemberSmith, Philip N.
dc.contributor.committeeMemberMayer, Gregory D.
dc.creatorSchmitt, Cassandra Denee
dc.creator.orcid0000-0002-5573-471X
dc.date.accessioned2019-11-01T15:00:45Z
dc.date.available2019-11-01T15:00:45Z
dc.date.created2019-08
dc.date.issued2019-08
dc.date.submittedAugust 2019
dc.date.updated2019-11-01T15:00:46Z
dc.description.abstractOrganophosphates (OP) are a class of compounds utilized as insecticides for agricultural application in the United States. Effective at controlling such insect populations as mites, aphids, and caterpillars, OPs have greatly contributed to increased crop productions since their early 20th century development. OPs are toxic to many non-target organisms through their primary mechanism of action as acetylcholinesterase (AChE) inhibitors. Precipitation often facilitates the distribution of OPs which contaminates surface waters. These insecticides are inadvertently introduced to aquatic ecosystems, recreational areas, and freshwater sources. Zebrafish (ZF) are a useful for investigations into the potential adverse effects of OP exposure, because they are a complimentary vertebrate model for the study of human health. The neurobehavioral and molecular alterations in embryo-larval ZF post exposure to OPs are well documented, but few studies observe the comparative effects across multiple compounds or generations. This thesis research comprises the comparative analyses of four predominant OPs (diazinon, dichlorvos, methyl-parathion, and dichlorvos) and a transgenerational examination of the OP metabolite chlorpyrifos-oxon developmental exposure in wild-type ZF. Investigations included a combination of neurobehavioral, biochemical, molecular, and epigenetic endpoints to assess both cholinergic and noncholinergic effects. This research contributes to previously limited data on the comparative and long-term effects of prenatal OP exposure in controlled laboratory conditions.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/2346/85339
dc.language.isoeng
dc.rights.availabilityUnrestricted.
dc.subjectOrganophosphates
dc.subjectInsecticides
dc.subjectZebrafish
dc.subjectDanio rerio
dc.subjectChlorpyrifos
dc.subjectDiazinon
dc.subjectMalathion
dc.subjectDichlorvos
dc.subjectParathion
dc.subjectTransgenerational
dc.subjectNeurodevelopmental
dc.subjectBehavior
dc.subjectMolecular
dc.subjectEnzymatic
dc.subjectEpigenetic
dc.subjectInheritance
dc.subjectGene expression
dc.titleExploration into the developmental toxicity of organophosphate insecticides in zebrafish (Danio rerio)
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentEnvironmental Toxicology
thesis.degree.disciplineEnvironmental Toxicology
thesis.degree.grantorTexas Tech University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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