Identification of bZIP Transcription Factors That Regulate the Development of Leaf Epidermal Cells in Arabidopsis thaliana by Single-Cell RNA Sequencing

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dc.creatorWu, Rui
dc.creatorLiu, Zhixin
dc.creatorSun, Susu
dc.creatorQin, Aizhi
dc.creatorLiu, Hao
dc.creatorZhou, Yaping
dc.creatorLi, Weiqiang
dc.creatorLiu, Yumeng
dc.creatorHu, Mengke
dc.creatorYang, Jincheng
dc.creatorRochaix, Jean David
dc.creatorAn, Guoyong
dc.creatorHerrera-Estrella, Luis (TTU)
dc.creatorTran, Lam Son Phan (TTU)
dc.creatorSun, Xuwu
dc.date.accessioned2024-05-14T18:05:43Z
dc.date.available2024-05-14T18:05:43Z
dc.date.issued2024
dc.description© 2024 by the authors. cc-by
dc.description.abstractEpidermal cells are the main avenue for signal and material exchange between plants and the environment. Leaf epidermal cells primarily include pavement cells, guard cells, and trichome cells. The development and distribution of different epidermal cells are tightly regulated by a complex transcriptional regulatory network mediated by phytohormones, including jasmonic acid, and transcription factors. How the fate of leaf epidermal cells is determined, however, is still largely unknown due to the diversity of cell types and the complexity of their regulation. Here, we characterized the transcriptional profiles of epidermal cells in 3-day-old true leaves of Arabidopsis thaliana using single-cell RNA sequencing. We identified two genes encoding BASIC LEUCINE-ZIPPER (bZIP) transcription factors, namely bZIP25 and bZIP53, which are highly expressed in pavement cells and early-stage meristemoid cells. Densities of pavement cells and trichome cells were found to increase and decrease, respectively, in bzip25 and bzip53 mutants, compared with wild-type plants. This trend was more pronounced in the presence of jasmonic acid, suggesting that these transcription factors regulate the development of trichome cells and pavement cells in response to jasmonic acid.
dc.identifier.citationWu, R., Liu, Z., Sun, S., Qin, A., Liu, H., Zhou, Y., Li, W., Liu, Y., Hu, M., Yang, J., Rochaix, J.-D., An, G., Herrera-Estrella, L., Tran, L.-S.P., & Sun, X.. 2024. Identification of bZIP Transcription Factors That Regulate the Development of Leaf Epidermal Cells in Arabidopsis thaliana by Single-Cell RNA Sequencing. International Journal of Molecular Sciences, 25(5). https://doi.org/10.3390/ijms25052553
dc.identifier.urihttps://doi.org/10.3390/ijms25052553
dc.identifier.urihttps://hdl.handle.net/2346/97986
dc.language.isoeng
dc.subjectbZIP transcription factors
dc.subjectjasmonic acid
dc.subjectpavement cells
dc.subjectscRNA sequencing
dc.subjecttrichomes
dc.titleIdentification of bZIP Transcription Factors That Regulate the Development of Leaf Epidermal Cells in Arabidopsis thaliana by Single-Cell RNA Sequencing
dc.typeArticle

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